chymotrypsin mechanism

step 1

substrate binds and serine attacks carbonyl

step 2

oxyanion collapses which cleaves the peptide bond

step 3

the first product leaves

step 4

water binds the enzyme and attacks carbonyl (nitrogen attacks hydrogen on water and O-H bond attacks the carbonyl

step 5

oxyanion collapses and the negative oxygen goes down and attacks the hydrogen attached to the nitrogen. second product is released