forensic biology exam 2 class review questions

proteins absorb light at what wavelength

280 nm

which of the following is NOT a benefit of presumptive serology tests

they can conclusively identify a biological fluid

in the Kastle-Meyer test, what chemical is used along with hydrogen peroxide to indicate the potential presence of blood?

Phenolphthalein

what enzyme in saliva is detected by the Phadebas test?

alpha-amylase

which of the following is NOT a general purpose required of all DNA extraction methods

separation of male and female components of cellular mixtures

what is the purpose of SDS in a DNA extraction lysis buffer

SDS is an ionic detergent used to break down the lipids in cell membranes

chelex protects DNA during the extracting process because

it binds to metal ions such as magnesium which are necessary for nuclease activation

what would happen if you forgot to add your chaotropic salt to the lysis buffer in a solid-phase extraction?

DNA would not bind in the solid-phase

why do we quantify our DNA

we need to know how much DNA is in our sample so we can have it in the appropriate range

a qPCR method that uses hydrolysis probes to detect each new strand of DNA is called the BLANK assay

Taqman

compared to the hydrolysis probe qPCR method, qPCR that uses SYBR Green intercalating dye is advantageous because 

the assay design is simple and little optimization is required

which of the following are advantages of using STRs for forensic DNA profiling?

STR have low mutation notes