Chapter 3 important terms

Specimen collection

Technique where a sample of interest is collected for analysis

Isolation

Creates colonies that each contain a single type of microbe import in identifying the exact species of microbes in a sample to eventually make a pure culture

Colony

If an individual bacterial cell is separated from other cells and has space and nutrients, it will grow into a mound of cells a- consisting of one species

Inoculation

Technique where a sample of microbesis introduced into a culture medium to allow the microbes to grow and multiply

Culture medium

A specially prepared substance ( liquid, solid, or semi-solid) designed to support the growth of microbes

Colony

Group of bacteria made up of one species

Positive stain

Stain that adheres to the cell and colors it

Negative stain

Stain repelled by the cell, coloring the background instead of the specimen

Simple stains

One dye is used; reveals shape, size, and arrangement

Differential stains

Use a primary stain and a counterstain to distinguish cell types or parts

Incubation

Inoculated media are placed in a controlled environment to promote growth, allowing microbes to replicate and increase visibility

Purpose of Streak plate technique

To isolate bacterial colonies where a sample is progressively diluted across a plate

Magnification

Ability to enlarge objects

Resolving power

Ability to show detail

Parts of the microscope

Compound microscope, two oculars for viewing, 4 objective lenses, mechanical stage for moving the specimen, lamp