Chapter 2-7: Study of Anaerobic Jars and Microbial Growth in Economics

to study obligate anaerobes, microaerophiles, and capnophiles

what are anaerobic jars used for

microbe that thrives in high CO2 concentration

what is a capnophile

BD GasPak EZ Anaerobic Jar system

what kind of anaerobic jar did we use

- ascorbic acid (Vitamin C) reacts with O2 gas to form dehydroascorbic acid and water (resulting in <1% O2 in the sealed jar)

- ascorbic acid goes from reduced state to oxidized state taking the O2 has out of the atmosphere of the jar

- GasPak contains inorganic carbonate which reacts to produce 13% CO2

describe how the BD GasPak EZ Anaerobic Jar system works

21% O2 and 0.04% CO2

state the concentrations of O2 and CO2 in the air we breathe

no it is non-catalytic

is the jar system catalytic?

oxyrase method - oxyrase is an enzyme that breaks down O2

give an example of a catalytic anaerobic jar system

methylene blue (we used this) and resazurin

give 2 examples of redox indicators used for anaerobic jars

- they are colorless when reduced and turn a color (methylene blue - blue, resazurin - pink) when oxidized

- placed as strips inside the anaerobic jar as experimental controls

state how methylene blue and resazurin work as redox indicators

Camplyobacter jejuni

what is the name of the pathogenic microaerophile that is a capnophile

Mannheima succiniciproducens - a bovine ruminant microbe (lives in the rumen of animals that chew cud such as cows or sheep)

what is the only microbe known that is an obligate capnophile

1. Alcaligenes faecalis because it has the most growth (obligate aerobe)

2. Clostridium sporogenes because there is no growth (obligate anaerobe)

3. Staphylococcus epidermidis because there is some growth (facultative anaerobe)

outside of jar:

give what microbe each number represents and why

1. Alcaligenes faecalis because there is no growth (obligate aerobe)

2. Clostridium sporogenes because there is lots of growth (obligate anaerobe)

3. Staphylococcus epidermidis because there is some growth (facultative anaerobe)

inside of jar:

give what microbe each number represents and why