Molecular genetics Exam 2

What happens if the replication fork catches up to a polymerase that is trying to transcribe?

They are able to pass through each other and carry on replication and trancription.

What happens if the replication fork collides HEAD ON with the polymerase trying to transcribe?

There will be a pause in replication and transcription, and a repair function will come in and allow replication to continue.

Why is it rare for a bacterial chromosome to have the issue of the replication fork and the polymerase trying to transcribe colliding HEAD INTO one another?

Because as replication and transcription occur, they are going in the same direction which is away from the origin or replication.

What is bacterial DNA’s go signal to start replication?

When both DNA strands are methylated

In bacterial DNA, when the DNA is replicated both strands are NOT methylated: True or False

False: the original strand is still methylated but the daughter strand is not methylated

What does bacterial DNA replication result in (think methylation)?

Hemi-methylation DNA where the parent strand remains methylated however the new strand is not methylated

How does Bacteria control the rate at which replication happens?

Through methylation. If one strand is methylated and the other one is not, then replication will not occur halting the rate of replication

Replication is NOT semi-conservative: True or False

False: Replication is semi-conservative

When Prokaryotic cells initiate replication, what is the first thing that happens?

The pre-replication complex binds to the OriC, creating a replication bubble which separates the DNA

What is the DnaA box in bacterial DNA sequences and what is around it?

It is the location where the DnaA protein binds and methylated flanking sequences surround it

What is the initiation protein for bacterial replication?

DnaA protein

Hemi-methylated DNA can continue into replication: True or False

False: Hemi-methylation prevents replication

Do we NEED fully methylated DNA for replication?

No we do not need it we just cannot have partially methylated DNA