anaerobic cultures

what are the three ways anaerobes can cause infection

1) increase in numbers in areas where they are part of the normal microbiota, 2) they are introduced into a new site 3) when a non normal microbiota anaerobic bacteria enters the host via penetrating wounds as a result of trauma

what plates should be used for an anaerobic culture

TSA, MAC, CNA, Choc, CDC, Ana CNA, Reduced BHI broth

what is chocolate plates used for in anaerobic cultures

aerotolerance testing

how long does it take for oxygen to kill fusobacterium,, anaerobic cocci, porphymonas, prevotella

10 minutes

what temp are anaerobic cultures kept at

37C

what are the best/perfect specimens for anaerobic cultures

aspirate obtained by needle or syringe, tissue and biopsy samples

What swabs are acceptable for anaerobic cultures

Eswab, anaerobic star swabs, Charboal swabs

which swab is specifically designed to maintain the viability of anaerobes, anarobes, and fastidious organisms

Esswab

how long should intrauterine devices be incubated for due to Actinomyces spp

10-14 days

what are four way the anaerobic enviornment is kept controlled

catalyst, dessicant, anatox, indicator strips

what is the atmospheric content of the anaerobic chamber

5% hydrogen, 5% CO2, 90% Nitrogen

what is the manometer

it is a gauge that monitors the air pressure inside the anaerobic chamber with a bubble indicator

What is important to check if a swab is received for an anaerobic sample

the swab needs to be moist! if not reject

are sputum samples acceptable for anaerobic cultures

no

are vaginal/cervical swabs acceptable for anaerobic culture

no

are BAL collections acceptable for anaerobic culture

no

are rectal or bowel samples acceptable for anaerobic culture

only if the source is specified as an abcess

what note needs to be added to refrigerated samples for anaerobic cultures

"culture results may be affected due to refrigeration

what is a commonly encountered anaerobe that stains as large spore producing GPB

Clostridium pergringes

what is a commonly encountered anaerobe that is known for double zone of hemolysis on BAP

Clostridium perfringes

what is a commonly encountered anaerobe that is the most common cause of gas gangrene infection

Clostridium perfringes

What common anaerobe needs to be ruled out and reported in all anaerobic cultures

Clostridium perfringes

what gram stain does Cutibacterium acnes have

GPB

what is a commonly encountered anaerobe that is a common skin contaminant

Cutibacterium acnes

what is a commonly encountered anaerobe that is a concern for infection in surgical infections, infect prosthetic joints, and devices

Cutibacterium acnes

what is a commonly encountered anaerobe spp that appears as a GPC in chains, clusters, pairs, or tetrads

Peptostreptococcus spp

What species includes Finegoldia, Anaerococcus, Parvimonas, Peptoniphulis, and Atopobium spp

Peptostreptococcus

what is a commonly encountered anaerobe that can cause infections in any part of the body

Peptostreptococcus spp

what is a commonly encountered anaerobe that appears as a long, thin, whispy GNB

Fusobacterium

what is a commonly encountered anaerobe that can be dry, “bread crumb”-Like on agar

Fusobacterium

what is a commonly encountered anaerobe that causes Lemeirre’s syndrome

Fusobacterium

What are the most common spp of Fusobacterium

necrophorum, nucleatum

what is a commonly encountered anaerobe spp that includes Fragilis, Thetamicron, Pyogenes, Vulgartus, Ovatus

Bacteroides

what is a commonly encountered anaerobe that appear as GNB

bacteroides

what is a commonly encountered anaerobe that is a large component of normal gut microbiota but cause infections if displaced in the body

bacteroides

what is a commonly encountered anaerobe that are aerotolerant

bacteroides

what is a commonly encountered anaerobe that stains as a GNB or coccobacilli

Prevotella

what is a commonly encountered anaerobe that includes melananinogenica and bivia as the most common spp

Prevotella

what is a commonly encountered anaerobe that are a large component of normal gut microbiota but cause infections if displaced in the body

Prevotella

what is a commonly encountered anaerobe that stain as GNC

Veillonella

what is a commonly encountered anaerobe that are normal oral flora that rarely causes infections

Veillonella

what is a commonly encountered anaerobe that includes common spp Atypica and Parvula

Veillonella