6.3d) – recombinant dna tech process
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Last updated 12:38 PM on 3/21/26
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5 Terms
1
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recom dna 1.
target gene isolated and amplified by pcr, taken out by restriction enzyme
2
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recom dna 2.
bacterial plasmid is removed using restriction enzymes
3
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recom dna 3.
sticky ends of target gene and bacterial plasmid connected via base pairing
4
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recom dna 4.
dna fragments joined by dna ligase to form circular plasmid or linear molecule
5
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recom dna 5.
plasmid inserted back into bacterial cell where multiple copies can be produced
