Untitled Flashcards Set

What is the selective ingredient in the EMB Test?

Dyes

What type of bacteria does the EMB select for?

Gram negative bacteria (coliform)

What type of metabolism occurs in an EMB test?

Fermentation

What sugars are fermented in an EMB test?

Lactose and sucrose

What is the purpose of the dyes in the agar of an EMB test?

To prevent growth of gram positive bacteria

What are fecal coliforms?

Bacteria that are found in human colon and stool samples

What is the EMB test useful for?

Helps see if water has been contaminated by sewage

What are the carbon sources in an EMB test?

Lactose or sucrose

What bacteria can tolerate the dyes but not ferment lactose or sucrose in EMB test?

Salmonella typhimurium

What bacteria agar lavender in an EMB test?

Salmonella typhimurium

What bacteria can tolerate dyes and ferment lactose or sucrose in EMB test?

Klebsiella aerogenes

What bacteria agar is dark purple in EMB test?

Klebsiella aerogenes

What bacteria tolerates dyes and strongly ferments lactose or sucrose in EMB test?

E coli

What bacteria agar is metallic green in EMB test?

E coli

What does the HE test help identify?

Bacteria that cause food poisoning

What is the selective ingredient in HE test?

Bile

What type of metabolism happens in HE test?

Fermentation

What sugars does the agar contain in the HE test?

Lactose, sucrose, and salicin

What bacteria can tolerate bile and ferment sugars in HE test?

E coli

What bacteria turns orange on agar in HE test?

E coli

What bacteria can tolerate bile and not ferment sugar or reduce sulfur in HE test?

Shigella flexneri

What bacteria turns blue/green in HE test?

Shigella flexneri

What bacteria can tolerate bile, reduce sulfur, and does not ferment sugars in HE test?

Salmonella typhimurium

What bacteria turns blue/green + black in HE test?

Salmonella typhimurium

What chemical reaction must take place for hydrogen sulfide to react with ferric ammonium citrate in HE test?

Reduction of sulfur

What sugar is used in the OF test in lab?

Glucose

What is the result of anaerobic metabolism in OF test?

Green on top chamber and yellow on bottom chamber

What is the result of aerobic metabolism in OF test?

Yellow on top chamber and green on bottom chamber

What is the result of facultative metabolism in OF test?

Yellow on both chambers

What metabolism occurs in E Coli in OF test?

Facultative metabolism

What metabolism occurs in Pseudomonas aeruginosa in OF test?

Aerobic metabolism

What sugar is used in lab for PR test?

Glucose

What is the purpose of the Durham tube in PR test?

It shows and forms bubble if bacteria can produce a gas

What is the pH indicator in PR test?

Phenol red

What color will the broth be if the bacteria can ferment sugar producing acid in PR test?

Yellow/orange and A+

What color will the broth be if the bacteria can not ferment sugar or produce acid in PR test?

Red and A-

What will happen in the tube if the bacteria can produce gas in PR test?

Bubble and G+

What will happen in the tube if the bacteria cannot produce gas in PR test?

No bubble and G-

What bacteria can ferment sugar producing acid and produce gas in PR test?

E coli A+/G+

What bacteria can not ferment sugar or produce acid or gas in PR test?

Enterococcus faecalis A-/G-

What pH does the citrate test for?

Basic pH

A positive citrate test is able to?

Import and utilize citrate from environment turning blue

A negative citrate test is able to?

Not import and utilize citrate from environment staying green

What bacterias have a positive citrate test?

Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella

What bacteria have a negative citrate test?

E coli

What makes the bacteria red in motility test?

TTC

What structure is being tested for in motility test?

Flagella

What bacteria is not motile and does not have flagella?

Klebsiella pneumoniae

What bacteria is motile and has flagella?

Proteus mirabilis

What bacteria contains urease and hydrolyzes urea turning bright pink in Urease test?

Proteus mirabilis and Klebsiella pneumoniae

What bacteria does not contain urease in Urease test staying yellow/orange?

E coli, Enterococcus faecalis, and Pseudomonas aeruginosa

What enzyme is tested in the Urease test?

Urease

What is important about the Urease test?

It does not tell you whether someone has a UTI; it tells you what might be causing the UTI

What does the S stand for in SIM Test?

Sulfur reduction

What does the I stand for in SIM test?

Indole production

What does M stand for in SIM test?

Motility

What color will the agar be if sulfur reduction occurs in SIM test?

Dark

What color will the agar be if no sulfur reduction occurs in SIM test?

Pale yellow

What color will the reagent layer be if the bacteria contains tryptophanase and produces indole?

Red

What color will the reagent layer be if the bacteria does not contain tryptophanase?

Colorless

What bacteria can reduce sulfur and does not contain tryptophanase?

Proteus mirabilis S+/I-

What bacteria cannot reduce sulfur and does not contain tryptophanase?

Klebsiella aerogenes S-/I-

What bacteria cannot reduce sulfur but contains tryptophanase producing indole?

E Coli S-/I+