Biomedical Science
Link Method
Used to find associations between evidence. Investigators evaluate the scene and then proceed through the area in a systematic and logical fashion
Line (Strip) Method
Used on large outdoor crime scenes. Members of the search team are arranged at intervals and search along straight lines
Grid Method
Used on large outdoor crime scenes. Searchers follow the first line pattern, once that is complete, they follow on the other line pattern
Zone Method
Used on crime scenes that are comprised of readily definable zones, such as houses or buildings. Teams are assigned in small zones for searching, the other searching methods are employed in each zone
Spiral Method (Inward or Outward)
Used on crime scenes with no physical barriers, such as open water. Can either being at critical point of crime scene (outward spiral) or the outer edge of the crime scene (inward spiral)
Wheel or Ray Method
Used on small circular crime scenes. Investigators start at a critical point and travel outward along many straight lines from this point
What are the steps to do at a crime scene?
1) Secure the scene
2) Witnesses (Identify, Separate, Interview)
3) Protect clothing/tools
4) Document (written description, photograph, sketching, observing)
5) Collecting samples/evidence
6) Analyze evidence
When what happens when you lie? (at least 3)
1) Heart beats faster
2) Warm body feeling
3) Emotions
4) Stutter
5) Relive the moment
6) Deliberate thinking
7) Fidgeting/shaking
8) Eye contact
9) Awkward laughter
10) Goosebumps
11) Sweating
What is measured on a Polygraph test?
1) Heart rate
2) Respiration rate
3) Blood pressure
4) Skin conductivity (sweat contains salt, salt conducts electricity)
AI: Body posture and Eye movement
How do vitals change during a lie?
1) Blood pressure spikes
2) Respiration rate drops/Heart rate spikes
3) Respiration rate increases/Heart rate drops
4) Sweating - most effective
Plain Arch
Arches slope upward then down. The simplest fingerprint.
Tented Arch
Similar to plain arch except the ridges in the center arch coverage thrust upward making a pitched appearance
Loop Pattern
Forms when ridges curve back on themselves
2 types: radical loop (pointing towards the thumb) ulnar loop (point towards the pinky)
Whorl Pattern
Whorls form a circular or spiral
Minutiae
tiny fingerprint ridge details
Occam’s Razor
If there are 2 possible explanations for an event, the one with fewest assumptions is usually correct
Cortex (Analyzing hair samples)
Most dense layer that contains majority of pigment when comparing thickness, color, granules
Cuticle (Analyzing hair samples)
Outermost layer characterized by scaled surface
What are the 4 main blood types?
Type A
Type B
Type AB
Type O
Why are there different blood types?
The difference is due to the presence or absence of particular protein molecules located on the surface of red blood cells
Antigens
surface proteins on red blood cells, a specific type of protein that causes an immune response
What type of antigen does type A blood have?
Type A antigens
What type of antigen does type B blood have?
Type B antigens
What type of antigen does type AB blood have?
Type A and Type B antigens
What type of antigen does type O blood have?
Neither A nor B antigens
Antibodies
A type of protein that circulates in your blood and interact with specific antigens
Aggulation
When antibodies bind to blood cells causign a clumping reaction
Human blood is composed of what 3 main blood cells?
1) Erythrocytes
2) Leukocytes
3) Thrombocytes (platelets)
What does cytes mean?
cell
Presumptive Tests
Kastic-Meyer (hemostick)
Liminol
Confirmatory Tests
ABO Testing
What are Erythrocytes also known as?
Red blood cells
What are Erythrocytes
a blood cell that contains a protein called hemoglobin
What do Erythrocytes do?
They carry oxygen from the lungs to all parts of the body.
What are Leukocytes also known as?
White blood cells
What are Leukocytes apart of?
They are a part of the immune system
What do Leukocytes do?
They protect your body from infection
What are Thrombocytes also known as?
Platelets
What are Thrombocytes?
a blood cell that controls blood clotting and healing
What do Thrombocytes do?
They control blood clotting, help wounds heal, and stops bleeding
What is the difference between an Antigen and an Antibody?
Antigens are protein that causes an immune response, and antibodies are a protein that interacts and binds to specific antigen
How many copies of genes do you have?
2 copies of every gene
one from mother, one from father
What blood type is dominant?
A and B
What blood type is recessive?
Type O
Homozygous
the same (IA IA)
Heterozygous
different (IB Ii)
Blood Splatter
when there is an injury, the pattern the blood leaves on surfaces tells a story
Transfers (Blood stain pattern)
occurs when a blood source comes into contact with a surface (smears, smudges, bloody fingerprints)
Splatter (Blood stain pattern)
produced when a source of liquid blood travels through the air before landing on a surface (like splatters)
Passive (Blood stain pattern)
When a droplet of blood is dropped directly from above and strikes a horizontal surface at 90°, it produces a circular stain.
What does it mean when spatters with an elliptical or elongated shape struck the surface at an angle.
This often means that an external force was applied that provided horizontal velocity, speed in a given direction, to the droplets
The more elongated the droplet
the faster they were moving
What does the long tail of each droplet indicate?
The long tail of each droplet indicates the direction it was moving when it struck the surface
What does DNA stand for?
Deoxyribonucleic Acid
What are the 4 nucleotides DNA is made of?
Adenine, Thymine, Guanine, Cystine
How many complementary strands are in a double helix?
2
If one strands sequence is AGGCTAC then the other strands sequence is
TCCGATC
What is a nucleotide made of?
Phosphate, sugar, and base
Hydrogen bonds
weak attractive force that holds nucleotide strands together
Sections of DNA that code for proteins
Genes
How many chromosomes do humans have?
46
How many chromosomes do humans get from their mother?
23
How many chromosomes do humans get from their father?
23
How many chromosomes in the human body are autosomes?
44
How many chromosomes in the human body are sex chromosomes?
2
What are the 3 parts of DNA fingerprinting?
1) Amplify the DNA
2) Cut the DNA
3) Separate the fragments
What does RFLPs stand for?
Restriction Fragment Length Polymorphism
PCR Amplification
makes millions of copies of DNA (polymerase chain reaction)
Restriction Enzymes Digestion
enzymes taken (originally) from bacteria cut DNA at VERY specific sequences
Gel Electrophoresis
When DNA samples are put into wells (holes) in a gel fin electrical current is applied to see where DNA bonds appear
What type of charge does DNA have?
DNA has a negative charge
How are DNA fragments separated?
separated based on size