Lab Homework #4- Total Protein, Packed Cell Volume Measurement and Reticulocyte Counts (Manual Method)

The PCV of Faith, the springer spaniel, is 16%, the Hgb is 2.25 g/dL and the RBC count is 5.7 X 10^6 /uL. Is she anemic? If so, how could you determine if it was a regenerative or a non-regenerative anemia? (List 3 different ways)

Red Blood Cell Indicies (MCV, MCH, MCHC), counting the reticulocytes on blood stain, and the big 6 (MAKE SURE YOU LIST THEM!!)

Using the following laboratory data calculate the reticulocyte percentage and the absolute reticulocyte count. (Show your work, round reticulocyte percentage to the nearest tenth, round absolute reticulocyte count to the nearest whole number, and use proper units)
Determine whether the anemia is regenerative or non-regenerative.
Feline
Red Blood Cell Count = 1.25 x 106 /uL
237 reticulocytes counted out of 1025 red blood cells

Reticulocyte Percentage

237 * 100/ 1025= 23.1%

Absolute Reticulocyte

23.1% * 1250000/100= 288,750 uL

Regenerative anemia

A reticulocyte will appear as what on a Wright’s stained blood film? 

polychromatophil

Using the following laboratory data calculate the reticulocyte percentage and the absolute reticulocyte count. (Show your work, round reticulocyte percentage to the nearest tenth, round absolute reticulocyte count to the nearest whole number, and use proper units)
Determine whether the anemia is regenerative or non-regenerative.
Canine
Red Blood Cell Count = 3.65 x 106 /uL
76 reticulocytes counted out of 1000 red blood cells

Reticulocyte Percentage

76 * 100/ 1000= 7.6%

Absolute Reticulocyte Count

7.6% * 3650000/100= 277,400 uL

Regenerative anemia

Using the following laboratory data calculate the reticulocyte percentage and the absolute reticulocyte count. (Show your work, round reticulocyte percentage to the nearest tenth, round absolute reticulocyte count to the nearest whole number, and use proper units)
Determine whether the anemia is regenerative or non-regenerative.
Canine
Red Blood Cell Count = 3.98 x 106 /uL
9 reticulocytes counted out of 1000 red blood cells

Reticulocyte Percentage

9 * 100/1000= 0.9%

Absolute Reticulocyte Count

0/9% * 3980000/100= 35,820 uL

Non-Regenerative anemia

How long after the onset of an anemia may it take for a reticulocytosis to be evident?

3-5 days

Why are reticulocyte counts performed?

Reticulocytes are quantified to help determine the bone marrow response to anemia (non-regnerative or regenerative).