DNA experiment- LC Biology

What is the aim of this experiment?

To isolate DNA from a plant tissue

What are the materials required?

• Onom

• Washing up liquid

• Blender

• Distilled water

• Sharp knife

• Table salt

• Protease enzyme trypsin

• Coffee filter paper

• Ethanol at freezer temp

• Droppers

• Water bath

• Spatula

• Ice water bath

• Plastic syringe

• 2 beakers

• Radiated

• Boiling tube and test tube rack

• Graduated cylinder

• Retort stand

• Glass rod

• Balance

• Funnel

How do you start the experiment

Add 3g table salt to 10cm cubed of washing up liquid in a beaker and fill up to 100cm cubed with distiller water

What do you add to the salty washing up solution?

Chopped up onion

Where should you place the beaker after adding in the onion?

After bath at 60 degrees for exactly 15 minutes

How do you cool the mixture after the water bath?

Stand the beaker in an ice bath for 5 minutes stirring frequently

How long should you blend the cooled mixture for?

No longer than 3 seconds

How much filtrate should be added to the boiling tube?

10cm cubed

What do you add to the filtrate in the boiling tube?

2-3 drops of the enzyme protease

After you add the protease, how should you add the next chemical to the filtrate?

Trickle 10cm cubed of ethanol (freezer temp) down the side of the boiling tube - forms a layer on top of the filtrate

What do you do with the boiling tube after all chemicals are added?

Leave without disturbing for a few minutes

How do you draw DNA from the test tube

Using a glass rod