2 - RED CELL SUSPENSION

Red Cell Suspension

It is performed to remove interfering substances in the plasma

4-5%

Concentration of RCS

postzone

If concentration exceeds, then it would cause a

Red Cell Suspension

Provides the red cell antigen

Normal saline solution

replaces plasma

Hard spin

for packed RBCs

Soft spin

for harvesting platelets

For optimal antigen concentrations

To remove interfering substances in the plasma

For crossmatching and reverse blood typing

Purpose of RCS (3)

lavender

Perform the standard venipuncture procedure using the _ top vacutainer tube

3000 rpm, 5 minutes

Centrifuge the EDTA blood sample at _ RPM for _minutes

1.5 mL

When most of the serum is removed, using a disposable pipette, obtain a _ mL of red cells from the bottom of the patient's sample tube and transfer them in the tube labeled Washed Red Cells

isotonic saline, ½ inch

Forcefully add _ _ from the wash bottle to the Washed Red Cells tube until the liquid level is about _ inch from the top. Be careful not to contaminate the tip of the wash bottle dispenser with blood

3-4

Cover the tube with parafilm and gently invert _-_ times to mix

1, 3400

Centrifuge the tube for _ minute at _ rpm. After centrifugation, observe the supernatant, if it is clear, proceed with the procedure. If there is a slight tinge of red, do not proceed because the specimen is hemolyzed and is unacceptable

3

how many washes of RBC

formula for total volume of rbc

formula for amount of NSS

Tomato red

The color of the suspension should be