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All 7 required proteases and their specificities
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Trypsin
RN-1 = Arg/R, Lys/K (Positively charged residues); RN =/= Pro
Chymotrypsin
RN-1 = Phe/F, Trp/W, Tyr/Y (Bulky hydrophobic residues); RN =/= Pro
Elastase
RN-1 = Ala/A, Gly/G, Ser/S, Val/V (Small neutral residues); RN =/= Pro
Thermolysin
RN = Ile/I, Met/M, Phe/F, Trp/W, Tyr/Y, Val/V; RN-1 =/= Pro
Pepsin
RN = Leu/L, Phe/F, Trp/W, Tyr/Y; RN-1 =/= Pro
Endopeptidase
RN-1 = Glu/E
Cyanogen Bromide
RN-1 = Met/M
Arg/R, Lys/K
Trypsin
Phe/F, Trp/W, Tyr/Y
Chymotrypsin
Gly/G, Ala/A, Val/V, Ser/S
Elastase
Ile/I, Met/M, Phe/F, Trp/W, Tyr/Y, Val/V
Thermolysin
Phe/F, Trp/W, Tyr/Y, Leu/L
Pepsin
Glu/E
Endopeptidase V8
Met/M
Cyanogen Bromide