LECTURE 2: Difficulties Caused by Improper Fixation
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Last updated 8:13 AM on 5/20/25
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7 Terms
1
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Cause/s: Failure to mix or insufficient fixative
Difficulties: Failure to arrest early autolysis
2
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Cause/s: Wrong choice of fixative
Difficulties: Removal of substances soluble in fixing agent
3
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Cause/s: Incomplete washing of fixative
Difficulties: Presence of artefacts on tissue sections
4
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Cause/s: Incomplete fixation
Difficulties: Tissues are soft and feather-like in consistency
5
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Cause/s: Wrong choice of fixative
Difficulties: Loss or inactivation of enzymes needed for study
6
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Cause/s: Over fixation
Difficulties: Shrinkage and swelling of cells and tissue structure
7
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Cause/s: Prolonged fixation
Difficulties: Tissue blocks are brittle and hard
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