LECTURE 2: Difficulties Caused by Improper Fixation

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7 Terms

1
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Cause/s: Failure to mix or insufficient fixative

Difficulties: Failure to arrest early autolysis

2
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Cause/s: Wrong choice of fixative

Difficulties: Removal of substances soluble in fixing agent

3
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Cause/s: Incomplete washing of fixative

Difficulties: Presence of artefacts on tissue sections

4
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Cause/s: Incomplete fixation

Difficulties: Tissues are soft and feather-like in consistency

5
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Cause/s: Wrong choice of fixative

Difficulties: Loss or inactivation of enzymes needed for study

6
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Cause/s: Over fixation

Difficulties: Shrinkage and swelling of cells and tissue structure

7
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Cause/s: Prolonged fixation

Difficulties: Tissue blocks are brittle and hard