What is the order of structure from cell to organism?
\-Cells connect and form tissue
\-Tissue connects to form organs
\-Organs connect to form organ systems
\-Organ systems connect to form organisms
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Prokaryotic Cell Components
1. No nucleus or other membrane-bound organelle 2. Nucleoid region 3. Cell wall made of peptidoglycan used to maintain shape and prevent dehydration 4. Capsule made of polysaccharide 5. Flagella used for locomotion 6. Pili used to exchange genetic information during conjugation (genetic transfer through direct contact) 7. Fimbriae used to attach to host cell 8. Ribosome used for protein synthesis 9. Chromosomes (DNA) 10. Size .1-5.0 μm
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Eukaryotic Cell Components
1. Size 10 - 100 μm
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A microscope is
an instrument that magnifies an object
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What is a micrograph?
An photo taken using a microscope
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Magnification is
the process of enlarging an object in apperance
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Resolving power is
the microscopes ability to distinguish two adjacent objects
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Unified Cell Theory
1. One or more cells comprise all living things 2. the cell is the basic unit of life 3. New cells arise from existing cells
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Organelle
“little organ”
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Nuclear Envelope
membrane enclosing the nucleus. Protein lines pores allow material to move in and out. Ch
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Chromatin
DNA plus associated proteinsNu
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Nucleolus
condensed region where ribosomes are formed
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Peroxisomes
metabolizes wastecyt
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cytoskeleton microtubules
form the mitotic spindle and maintain cell shape
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centrosome
microtubule organizing centerinter
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intermediate filaments
fibrous proteins that hold organelles in place
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microfilaments
fibrous proteins that form the cellular cortex
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plasma membrane
Phospholipid bilayer with embedded proteins that separates the internal contents of the cell from its surrounding environment
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What is the role of the Lysosome?
* digests food and waste materials * destroy pathogens using hydrolytic enzymes
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What is a macrophage?
A group of white blood cells that are part of the body’s immune system.
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cytoplasm
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mitochondria
produce energy
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vacuole
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Rough Endoplasmic Reticulum (RER)
associated with ribosomes; makes secretory and membrane proteins
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Smooth Endoplasmic Reticulum (SER)
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Nucleus
Contains chromatin, a nuclear envelope, and a nucleus
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Plasmodesmata (plants)
Channels that connect two plant cells
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Central Vacuole (plants)
filled with cell sap that maintains pressure against cell wall
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Cell Wall (plants)
* Rigid covering that protects the cell, provides structural support and maintains cell shape * Primary component is cellulose.
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Plastid (plants)
Stores pigments
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Chloroplast (plants)
site of photosynthesis
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Phospholipid
lipid molecule with two fatty acid chains and a phosphate-containing groupGl
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Glycolipid
Lipid with carbohydrate attached
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Glycoprotein
Protein with a carbohydrate attached
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What does the plasma membrane control?
The passage of organic molecules, ions, water and oxygen in and out of the cell, as well as the exit of wastes such as carbon dioxide and ammonia.
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Microvilli
projections of the plasma membrane that increase the surface area available for absorption
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Cytoplasm
The cell’s region between the plasma membrane and the nuclear envelope. 70-80% water, semi-solid consistency,
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cytosol
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Nucleus
Houses the host cells DNA and directs synethesis of ribosomes and proteins.
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Nuclear Envelope
Double membrane structure that constitutes the nucleus’ outermost portion, both the inner and outer membranes are phospholipid bilayers
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Nucleoplasm
Semi-solid fluid inside the nucleus
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Chromosomes
Structures within the nucleus made up of DNA. Every eukaryotic species has a specific number of chromosomes in the nucleus of each cell.
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Chromatin
The unwound protein-chromosome complexes that makes up chromosomes when condensed and desondensed.
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Nucleolus
Aggregates ribosomal RNA with associated proteins to assemble ribosomal subunits.
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Ribosomes
* Responsible for protein synthesis. * Consist of two subunits; large and small * Attach to: Plasma membranes cytoplasmic side, ER’s cytoplasmic side, nuclear envelope outer membrane. * Freely float as single units or polyribosomes
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Polyribosomes
clusters of ribosomes
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How do ribosomes receive their orders?
1. DNA within the nucleus transcribes into messenger RNA (m-RNA) 2. m-RNA travels to ribosomes which translate the code 3. Ribosomes then assemble amino acids into proteins during protein synthesis
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Where are ribosomes particularly abundant?
Cells that synthesize large amounts of protein
* Pancreas - digestive enzymes * \
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Mitochondria
* Responsible for making adenosine triphosphate, the cells main energy carrying molecule * Oval shaped, double membrane organelles * Contain separate DNA and ribosomes * Each membrane is a phospholipid bilayer embedded with proteins. * Inner folds called cristae which increase surface area * Area inside cristae is the matrix
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What is ATP (Adenosine Triphosphate)?
The cells main energy carrying molecule. ATP represents the cell’s short term stored energy.
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What is cellular respiration?
* The process of making ATP using the chemical energy in glucose and other nutrients. * Uses oxygen and produces carbon dioxide as a waste product.
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Which cells have a high concentration of mitochondria?
Muscle cells, because muscles require a lot of energy.
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What happens when cells don’t get enough oxygen?
They do not produce much ATP, and instead produce lactic acid in the absence of oxygen.
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Peroxisomes
* Small round organelles enclosed by single membranes * Responsible for breaking down fatty acids and amino acids using oxidation reactions * Detoxify poisons * Releases hydrogen peroxide (H2O2), but contains enzymes that safely break down H2O2. * \
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Glyoxysomes
Specialized peroxisomes in plants that convert stored fats into sugars
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Vesicles
* Membrane-bound sacs that function in storage and transport * Does fuse with the membranes of other cellular components * \
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Vacuole
* Membrane-bound sacs that function in storage and transport * Does not fuse with the membranes of other cellular components
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Centrosome
* The organelle where all microtubules originate * Microtubule organizing center (MTOC) found near the nuclei of animal cells. * Contains a pair of centrioles (perpendicular to each other) * Each centriole is a cylinder of nine triplets of microtubules held together by nontubulin
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Lysosomes
* The cells “garbage disposal”, using enzymes to break down proteins, polysaccharides, lipids, nucleic acids, and worn-out organelles. * Very acidic 4.5-5pH * \
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What is peptidoglycan?
Primary component of prokaryote cell walls.
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What is cellulose?
A polysaccharide comprised of glucose units.
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What is photosynthesis?
A series of reactions that use carbon dioxide, water, and light energy to make glucose and oxygen.
A series of interconnected membranous sacs and tubules that modify proteins and synthesize lipids
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Where does protein synthesis occur?
In the rough endoplasmic reticulum
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Where does lipid synthesis occur?
In the smooth endoplasmic reticulum.
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What is the ER tubules’ hollow portion?
Lumen or cisternal space
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What is the relationship of the ER and the nuclear envelope?
The ER’s membrane is continuous with the nuclear envelope.
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What happens to proteins in the RER lumen?
They undergo folding or acquire side chains.
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Where is the RER abundant?
In cells that secrete proteins such as the liver?
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What is the SER (smooth endoplasmic reticulum) function?
* Synthesis of:
1. Carbohydrates 2. Lipids 3. Steroid hormones
* Detoxification of medications and poisons * Storing calcium ions
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What is the sarcoplasmic reticulum?
A specialized smooth endoplasmic reticulum found in muscle cells responsible for storing calcium ions needed to trigger coordinated contractions.
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What is the golgi apparatus?
The organelle that tags, packs, and distributes lipids and proteins.
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What is the side of the golgi apparatus closer to the ER?
The cis face
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What is the side of the golgi apparatus farthest from the ER?
The trans face
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What is the most common modificaton that happens in the golgi apparatus?
The addition of short sugar molecule chains
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Where are golgi apparatuses most abundant?
* In secretory cells such as salivary gland cells * In cells that secrete digestive enzymes * In immune cells that secrete antibodies
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What is the additional role of the golgie apparatus in plant cells?
Synthesizing polysaccharides
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What is the process of phagocytosis?
1. Plasma membrane invaginates (folds in) to engulf a pathogen 2. The invaginated section pinches of and becomes a vesicle inside the macrophage 3. A lysosome fuses to the vesicle and uses hydrolytic enzymes to destroy the pathogen
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What is the role of the cytoskeleton?
1. Maintain cell shape 2. secure organelles in position 3. Allow cytoplasm and vesicles to move withing the cell 4. Enables multicellular organisms to move
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What three types of fibers make up the cytoskeleton?