Microbiology Lab 4 - Smear & Simple Staining Midterm Practice

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11 Terms

1
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Purpose of fixing a smear?

Kill bacteria and stick them to slide

2
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What is a heat fix?

flame or hot plate

3
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What is a chemical fix?

95% methanol

4
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What does simple stain show?

Cell shape, size, and arrangement.

5
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What is a chromophore?

Ion responsible for color of the dye

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Basic stain charge and examples?

Positive ion; Methylene Blue, Crystal Violet

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Negative stain charge and examples?

Negative ion; Eosin, Nigrosin

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Why use basic stains on bacteria?

Cell wall is negatively charged → attracts positive stain.

9
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Does simple stain differentiate species?

No. It’s not differential.

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One advantage of negative stain over simple?

Cells don’t shrink – true morphology seen.

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Do you heat-fix negative stains?

No