REQUIRED PRACTICAL: MICROSCOPY
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Last updated 10:41 AM on 11/19/22
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8 Terms
1
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HOW DO YOU PREPARE A MICROSCOPIC SLIDE
Peel off epidermal layer using forceps
Place sample on drop of water on microscope slide
Add drops of iodine solution to sample
Lower cover slip onto sample using mounting needle
Place sample on drop of water on microscope slide
Add drops of iodine solution to sample
Lower cover slip onto sample using mounting needle
2
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WHY IS A THIN SAMPLE OF TISSUE REQUIRED
Allows light to pass through so internal structures can be observed
3
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WHY IS WATER ADDED TO THE SLIDE BEFORE THE SAMPLE IS MOUNTED
Allows sample to be suspended between the slide and coverslip, ensures coverslip stays in place
4
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WHY IS IODINE SOLUTION ADDED TO STAIN THE CELLS
Iodine solution adds contrast allowing internal structures to become more visible
5
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WHY IS A MOUNTING NEEDLE USED TO LOWERED COVER SLIP
Ensures that no air bubbles are trapped
6
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HOW DO YOU MEASURE THE LENGTH OF A CELL VIEWED UNDER THE MICROSCOPE
Measure using eyepiece and calibrate with stage micrometer
7
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HOW DO YOU CALCULATE MAGNIFICATION OF AN IMAGE
Magnification = observed cell length / actual cell length
8
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WHAT SAFETY PRECAUTIONS SHOULD BE TAKEN DURING THIS EXPERIMENT
Wear safety goggles when handling iodine solution
