Lab Homework #3- Red Blood Cell Indices, Total Protein, Packed Cell Volume

You just obtained a PCV of 34% on Boo the dog. You estimate his hemoglobin value to be approximately:

11.33 g/dL

If you had a hemoglobin value of 2.5 g/dl on a horse you would expect the packed cell volume to be approximately:

7.5%

Estimate the hemoglobin on a bovine blood sample that has a PCV of 63%.

21 g/dL

Kristy was doing a quick PCV and TP for Dr. Funnell. She noticed that after she had spun the microhematocrit tube the plasma was milky red in color. She reported to Dr. Funnell that the PCV was 36%, the TP was 5.9 g/dl and that the plasma was:

hemolyzed and lipemic

Peter did a TP on Buck the Samoyed puppy. He noticed that the plasma had a slight yellow tinge to it. What is the term for this yellow tinge?

icteric

Plasma which is ______ and/or _____ may make it difficult to determine TP. TP may be elevated due to these changes.

hemolyzed or lipemic

I’m looking at a hemacytometer on the microscope and I can’t find anything. The lighting seems a bit bright. How do you recommend resolving this problem? Give at least two solutions. Use the proper name for the microscope part to receive full credit.

One way to fix this problem is to lower the condenser that is located right below the stage. The condenser allows further control of illumination of the specimen, as well as, enhancement of the resolution. It is very likely that the condenser is too condensed on the specimen and needs to be weakened for less illumination of the specimen. Another way to fix this problem is to lessen the amount of light entering the condenser by adjusting the field diagram lever. This will allow for less light to come through the condenser making it easier to see.

Using the following laboratory data calculate the MCV, MCH and MCHC on this dog sample and then describe the blood cell picture. (Example: normocytic, normochromic). Show your work and use proper units!
Total Protein = 7.1 g/dL
WBC Count = 9,200 / mm3
RBC Count = 7.64 x 106 / mm3
Hemoglobin = 16.1 g/dL
Hematocrit = 48 %

MCV

48 * 10 divided by 7.64 mm3 = 62.8 fL

MCH

16.1 g/dL * 10 divided by 7.64 mm3 = 21.1 pg

MCHC (normochromic)

16.1 g/dL * 100 divided by 48 = 33.5 g/dL

Identify the areas of this spun microhematocrit tube

1. Erythrocytes

2. Buffy coat

3. Plasma

Estimate the PCV of a ferret sample that has a hemoglobin of 18 g/dl

54%

Estimate the hemoglobin of a horse sample that has a PCV of 22%

7.33 g/dL

Using the following laboratory data calculate the MCV, MCH and MCHC on this dog sample, then describe the blood cell picture (example: normocytic, normochromic). Show your work and use proper units!
Total Protein = 7.3 g/dl
WBC count = 3,400 / mm3
RBC count = 2.15 x 106 / mm3
Hemoglobin = 5.0 g/dl
Hematocrit = 16%

MCV (macrocytic)

16* 10 divided by 2.15 mm3= 74.42 fL

MCH

5.0 g/dL* 10 divided by 2.15= 23.26 pg

MCHC (Hypochromic)

5.0 g/dL* 100 divided by 16= 31.25 g/dL