Ion Exchange Chromatography and Protein Analysis

These flashcards cover key concepts and details related to ion exchange chromatography, protein analysis, and specific experimental results.

What is the principle of separation in ion-exchange chromatography?

Molecules are separated based on charge.

Which types of molecules are separated using ion-exchange chromatography?

Molecules are separated based on their charge.

What major bands would a protein tetramer of 10 kD each show on an SDS-PAGE gel?

The gel would show major bands at 10 kD.

What type of bonds do proteins form in Immobilized Metal Affinity Chromatography (IMAC)?

Proteins form metal-ligand bonds.

If a protein has an isoelectric point (pl) of 3.2, which amino acids is it likely to have a lot of?

It is likely to have a lot of D and E.