Biochemical Testing

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15 Terms

1
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What occurs in a Catalase test?

  • Hydrogen peroxide (H2O2) forms

  • As a by-product of aerobic metabolism

2
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Which enzyme breaks down H2O2 into _

  • Catalase

  • Breaks down 2H2O2 → 2H2O (*water) + O2 (*oxygen)

<ul><li><p>Catalase </p></li><li><p>Breaks down 2H<sub>2</sub>O<sub>2</sub> → <u>2H<sub>2</sub>O (*</u><strong><u>water</u></strong><u>) + O<sub>2</sub> (*</u><strong><u>oxygen</u></strong><u>)</u></p></li></ul><p></p>
3
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Test used to detect the presence of the enzyme Catalase

Catalase Test

4
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What does Catalase Test help distinguish 

  • Gram+ cocci: Staphylococci and Streptococci

<ul><li><p><span>Gram+ cocci:&nbsp;</span>Staphylococci and Streptococci</p></li></ul><p></p>
5
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What does the presence of bubbling indicate?

Enzyme Catalase is present

<p>Enzyme Catalase is present </p>
6
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If Catalase Test Positive, could be what 2 bacteria?

  • Staphylococcus

  • Micrococcus

7
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If Catalase Test Negative, could be what 2 bacteria?

  • Enterococcus

  • Streptococcus

8
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The Oxidative Test detects the presence of _

bacteria that use Enzyme Cytochrome C Oxidase in cellular resp 

9
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What does Oxidative Test help distinguish 

Gram Negative Bacilli

10
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2 functions of Cytochrome C. oxidase

  • Final enzyme used in the electron transport chain

  • Transfers electrons from a molecule to oxygen

11
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The Oxidase Reagent is a _ 

Chromogenic reducing agent- chemical that develops color as

oxidized

12
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What reacts w cytochrome c oxidase & what color does it turn it 

  • Oxidase Reagent 

  • turns C Oxidase purple 

13
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If Oxidase Test Positive, could be what 2 bacteria?

  • Pseudomonadaceae

  • Neisseria

14
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If Oxidase Test Negative, could be what bacteria & 2 examples?

  • Enterobacteriaceae:

    • Escheriichia coli

    • Serratia marcescens

15
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What can’t be used in Oxidase test & why 

  • metal loop to transfer bacteria bc:

  • metal will react with oxidase reagent & give false + result

    • *result needs to be seen within 10s.