MLT 118 Exam 2

All are relative expression except:

Concentrated water

Saturated Solution

0.9% Saline

Super saturated water

0.9% saline

V/V W/V and W/W are what kind of expressions?

Quantitative Expressions (physical)

What is a system used to ensure the validity of overall analytic performance of lab procedures?

Quality Assurance

What is a substance or constituent in which the lab conducts tests?

Analyte

What is the procedural steps performed to determine the kind or amount of analyze in a specimen?

Analysis

What’s the name of a chemical that is used to convert the analyze from the sample into a measurable form? It can be a solution or powder.

Reagent

The action of assessing, setting of correcting a device by adjusting it to match or conform to a reliable, known measure is called…

calibration

What are the three types of analysis?

Qualitative

Semi-quantitative

Quantitative

Pregnancy test came back positive (+), this is a ____ result of analysis.

qualitative

The patients blood glucose results came back, a total of 300mg/dL, this is a ______ result of analysis.

quantitative

The protein and glucose on reagent strips are examples of what analysis? ( 1+, 2+. +/- )

semi- quantitative

The blood column on reagent test strips is an example of what analysis type?

qualitative

Pre-filtration, distillation, deionization, reverse osmosis, ultra filtration and nano-filtration are…

methods of water purification

What is number 1?

BEAKER

What is number 2?

ERLENMEYER FLASK

What is number 3?

VOLUMETRIC FLASK

What is number 4?

GRADUATED CYLINDER

Whats the name of this container in the image?

Florence flask

What are the correct names for glassware?

borosilicate and low actinic

What type of glassware has high degree of thermal resistance, low alkali content and free of heavy chemicals?

borosilicate

Which glassware has high thermal resistance, protects light-sensitive substances and is amber/red in color?

low actinic

A salt sample is placed into some water and all of it dissolves without stirring or heating. When you add another pinch of salt it continue to dissolve. The resulting solution is:

unsaturated

You have water(solvent) & acid(solute) need for the solution. Which goes first?

water

_________________is a type of dilution with multiple, progressive dilution processes that may be required for certain quantitative tests. 

Serial dilution or sequential dilution

Blood samples are usually spun in a ____________________to get serum or plasma.

centrifuge

All of the following are true of pipets EXCEPT:

All micropepettes measure the same volume.

Micropipets measure and deliver in microliter (µL).

Pipette calibration is done by Gravimetric procedure.  

Volumetric pipettes can be used to reconstitute lyophilized controls and calibrators

All micropepettes measure the same volume.

The speed of a centrifuge should be checked at least once every 3 months with a (n):

Potentiometer

Tachometer

Ergometer

Wiper

Tachometer

Which of the following is not %V/V (volume by volume) expression?

0.9% Saline

70% Ethanol

60% Isopropanol

10% Bleach

0.9% Saline

Which of the following does NOT require calibration in the clinical laboratory?

Erlenmeyer flask 

Centrifuge

Electronic balance

Micropipette

Erlenmeyer flask

Solution = _________________   +  ___________________

Solvent : Solute

There is water and salt in a container: define which is the solute and solvent.

water is solvent and salt is the solute

Solution properties that depend on the concentration of solute particles, not their identity are called…

Colligative properties

Glucose and creatinine are called what in clinical terms?

clinical analytes

Name 3-4 Clinical analytes.

glucose, creatinine, bilirubin, sodium, albumin protein and cholesterol

1. vapor pressure depression,

2. boiling point elevation,

3. freezing point depression

4. osmotic pressure

These are all called…

the four colligative properties

Which of the following statements is true about colligative properties?

The more particles dissolved in a solution will make the solution's freezing and boiling points higher in temperature.

Increasing the number of particles dissolved in a solution will make the solution's boiling point extremely higher.

The more particles dissolved in a solvent the lower the solution's boiling point.

The greater the number of particles dissolved in a solution the lower the solution's freezing point.

The greater the number of particles dissolved in a solution the lower the solution's freezing point.

Is a mixture of two or more solids…

solid solution

A homogenous mixture of two ore more gases..

Gaseous solution

The solvent is always liquid; the solute can be any state of matter..

liquid solution

Which of the following is not relative expression of solutions? 

Diluted acetic acid

1M hydrogen peroxide

Concentrated urine

Saturated glucose

1M hydrogen peroxide

__________is a process by which the concentration of a given solution is decreased by the addition of solvents to make a weaker solution.  

Dilution

A type of dilution where one part of the original solution

simple dilution

A type of dilution using larger numbers; useful when the volume of concentrate or diluents is in short supply

serial dilution

what is this tool in the image and what’s its purpose?

rocker ; it is for mixing

Graduated cylinders:

are made only of plastic

are used by reading volume at meniscus

range in size from 5–50 mL

have a flared neck

are used by reading volume at meniscus

What form of temperature scale is used in clinical lab?

Celsius

A solution is made by mixing 70mL of alcohol and 30mL of water in a volumetric flask. The alcohol is referred to as the______________

solvent

solute

filtrate

solute

A noncritical measurement can be:

made using a volumetric flask

used when making a standard

made using an Erlenmeyer flask

made using a volumetric pipet

made using an Erlenmeyer flask

Look the below laboratory equipment and answer the following questions:

A) What is the range of volume measured by this equipment? ______________________

B) Can you use it to measure 50μl of sample? ________________________ 

A. 100-1000 microliter

B.NO

You have been instructed to create a working solution of HCl acid. You start to mix the working solution from a very concentrated stock solution of the acid. In what order should you complete this task?

__ Get a clean beaker

__ Carefully add acid slowly and in small amounts

__ Label the beaker clearly with the contents it will contain

__ Add the designated amount of water needed to make the working solution

1, 4, 2, 3

pH is defined as the negative logarithm of the ______________ ion concentration

Hydrogen, H

A proton donor which furnish hydrogen ions is called

an acid

A proton acceptor which furnish hydroxyl ions is called

a base