WBC

- Marrow <3%
- Blood none
- Nucleus is large, round or slt oval, with 2-5 nucleoli, and smooth fine chromatin
- cytoplasm is medium blue color with no granules
- Type I

myeloblast

- 15-25 um
- Marrow 1-5%
- Blood none

Promyelocyte

- Marrow 10-15%
- Blood none
- Has secondary (lilac) granules

neutrophilic myelocyte

- Size 10-15 um
- marrow 15-30%
- blood rarely

Neutrophilic metamyelocyte

Neutrophilic band

- size 10-15 um
- marrow 15-25%
- blood 50-70%

segmented neutrophil

What is the neutrophilic sequence?

granulocyte-monocyte progenitor → Myeloblast → Promyelocyte → Myelocyte → Metamyelocyte → Band → Segmented Neutrophil

What is the monocyte sequence?

granulocyte-monocyte progenitor → Myeloblast → Promyelocyte → Myelocyte → Metamyelocyte → Band → Segmented Neutrophil

Monoblast

Hint: nucleus is too convoluted to be a blast

Promonocyte

Bone marrow: <2%
Blood: <10%

Monocyte

Macrophage

What is the eosionophilic sequence?

Eosinophil-basophil progenitor → Myeloblast → Promyelocyte →eosinophilic myelocyte →eosinophilic metamyelocyte → eosinophilic band → eosinophil

Eosionophilic myelocyte

Eosinophilic metamyelocyte

eosinophilic band

- Size 12-17
- Blood 0-7%
- Abs <0.7 x 10^3/ul

eosinophil

what is the basophilic sequence?

Eosinophil-basophil progenitor → Myeloblast → promyelocyte → basophilic myelocyte →basophilic meta-myelocyte →basophilic band → basophil

- Size 10-15 um
- Blood 0-3%
- Abs. < 0.2 x 10^3/ul

basophil

Identify this inclusion and cell.
What causes this? What does it indicate?

Auer rod in a Myeloblast.

- caused by granules in the cell fusing together.
- Indicator of leukemia and that the cell is not maturing properly

What is the most important content of primary granules? What does it do?

Myeloperoxidase
- potentiates HOCl killing microbes

Size: 2-4 um
No nucleus
Light blue with red granules
Live for about 7-10days

Platelets

- Size 25-35 um

Megakaryoblast

Promegakaryocyte

Megakaryocyte

platelets

Bone marrow - not defined
Blood 0%
- the nucleoli are a flag for this cell and the outline of the cell has a crisp edge

- only seen if someone has leukemia

- hardest cell to identify because it is not much bigger than its mature kin (see slide 24 of hematopoiesis part 2)

lymphoblast

Bone marrow - Not defined
Blood - None

- Flag for this cell is that it has one large nucleolus in the center
- larger than its mature kin

- Mike Wazowski cells

Prolymphocyte

Bone marrow - 5-15%
Blood 20-40%

lymphocyte

Bone marrow - 0-1%
Blood - 0%
- rarely seen in blood

Plasma cell

5-20% of peripheral blood lymphs and count as a lymph (not any special reporting)

- have pink granules in them that are big enough to see
- Monocyte granules are not distinct enough to see them, that is how you tell the difference.

Natural killer (NK) cell

5-6% in normal blood
- Indicates functioning immune system
- See a lot in patients with EBV
- They get reported if >10 but usually lumped in with regular lymphs if only a few are seen

Variant lymph
(or atypical lymph or reactive lymph)

Precursor right before a plasma cell, does not meet all 4 characteristics of a plamsa cell
- reported as a variant lymph

Plasmacytoid

- A variant lymph

Mono-type lymph

a variant lymph

"Blast-like" lymphocyte

variant lymph

Flame cell

Bubbles are Abs being produced
- variant lymph

Mott cell or Morula cell