BIOL 2460 Lab final exam

what will you be using to demonstrate proper hand washing in lab 1

colored paint

which best describes proper aseptic technique

the proper use of lab protocols to maintain sterility to prevent contamination of a patient or surface

biohazardous waste like used bacteria cultures tubes should be disposed of in which container

test tube waste rack

you should wash your hands before and after completing work in the lab classroom

true

according to CDC how often should you wash your hands

20 seconds

you should label everything in the lab including petri dishes and test tubes. Which of the following should be included when labelling items

name

date

microorganism used

according to CDC you should apply water before applying soap when washing your hands

true

microbiology lab classrooms are considered

BSL-2

bacillus anthracis is considered a dangerous pathogen & must be handled in what lab

BSL-3

biohazardous waste like used petri dishes should be disposed in which container

red bin

trial 1 = 350ul

trial 2 = 375ul

trial 3 = 370ul

average = 365ul

standard deviation = 13.23ul

pipetting was not accurate or precise

how many microliters (ul) in 1 milliliter (ml)

1000

which is not required for BSL 2

biosafety cabinet with negative air pressure

after using your inoculating loop to gather E. coli bacterium, what should you do to ensure proper aseptic technique

pass inoculating loop through bunsen burner flame

which is used to assess minimum inhibitory concentration in lab 2

etest

MIC is abbreviation for what test

minimum inhibitory concentration

which is NOT a good example of aseptic technique while setting up disk diffusion assay

throwing used cell spreader into grey trash bin

which tool will NOT be used to set up kirby bauer assay

inoculating loop

precision is the ability to transfer a volume of liquid that is close to true or nominal value as indicated by pipette tool itself

false

the efficacy of a disinfectant can be assessed through ____ in a disk diffusion assay

zone of inhibition

examine MIC value

0.094

which medium selects for gram positive cocci such as staphylococcus aureus

mannitol salt agar

which is the best description of kirby bauer assay

a method that determine the susceptibility of specific microbes to various antimicrobial drugs impregnated on filter disk

which 2 lenses are used to calculate total magnification of a microscope

ocular lens & objective lens

examine MIC value part 2

0.75

examine which disinfectant is LEAST effective against microorganism

D

which disinfectant is MOST effective against microorganism

A

microorganism exhibits large zone of inhibition when incubated with azithromycin during kirby bauer assay

antibiotic is likely effective against the microorganism

term coccus used to refer microbes

cellular shape

immersion oil improves resolution by increasing

numerical aperture

best describe objective lens of microscope

is a rotating set of lenses on a microscope that have various magnification typically ranging from 4x to 100x

you are using a microscope with 40x. What is your total magnification

400x

what is the difference between cellular morphology & cellular arrangement

morphology refers to shape of individual microbial cell while arrangement refers to pattern that forms during cellular division wh

which is NOT a type of bacterial cellular morphology

tetrad

during the gram stain you can obtain a false negative by leaving the decolorizer on too long

true

which is NOT a category that the API test examines

lipid catabolism

which BEST describes anabolism

when small molecules are assembled into larger ones

some bacteria in enterobacteriaceae family are gram positive and some are gram negative

false

many members of enterobacteriaceae family have a ___ relationship with humans

mutualistic

which stain acts as the counterstain in the gram staining technique

safranin

plaque that can be found on teeth is an example of biofilm that has formed

true

antibodies are produced by

adaptive immune system

which is the first step in biofilm formation

reversible attachment of planktonic cells

almost half of the tests in the analytical profile index determine which type of metabolic process

fermentation

urease an enzyme to break down urea tissue invasion in pathogens such as proteus mirabilis. This breakdown of urea is considered ___ reaction

catabolism

which is NOT an element of an indirect ELISA test

decolorizing agent

ELISA assay are useful in detecting and quantifying biofilm formation

false

which ELISA reagent has a bound chromogenic molecule

secondary antibody

which is NOT an accurate hypothesis that explains antibiotic resistance developed from biofilms

cells found deep within the layers of biofilm are very metabolically active and may be less susceptible to antibiotics that disrupt metabolic activities

which is used in this lab to stain biofilm

crystal violet