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Direct Fecal Smear

A routine stool examination procedure useful for the detection of motile protozoan trophozoites.

pale; transparent

: In a direct saline preparation, trophozoites appear very _____ and _____.

Nair’s buffered methylene blue (BMB)

A solution used to stain trophozoites to demonstrate nuclear morphology.

pale blue; darker blue

When stained with BMB, the Entamoeba cytoplasm appears _____ while the nucleus appears _____.

Weak iodine solution

A temporary stain used in direct fecal smears to demonstrate the nuclei of protozoan cysts.

eggs; larvae

Besides protozoa, the direct fecal smear preparation can also detect helminth _____ and _____.

2mg

One fecal smear should ideally contain approximately _____ of stool.

very small amount of stool

Why might light infections be missed during a Direct Fecal Smear (DFS)?

Clean glass slides, applicator sticks, and normal saline solution (NSS).

List the three primary materials required for a saline fecal smear.

mucoid; bloody

Front: When collecting stool with an applicator stick, one should poke various portions, especially _____ and _____ areas.

Homogenous suspension

The result of spreading a fecal specimen over NSS while ensuring it does not run down the edges of the slide.

place slide ovber printed material if preparation is correct prints are barely readable

How is the correct thickness of a saline fecal smear verified?

morphologic details; ova

The Kato technique is used to enhance _____ and increase the chances of isolating _____ of different parasites.

Glycerin

A reagent used to clear fecal debris so that parasite ova become more visible during microscopic analysis.

Kato Katz

The _____ is a quantitative method that requires a template for accurate reporting.

Kato Thick

The qualitative method of the Kato technique

24hrs

: For the Kato technique, cellophane squares must be soaked in glycerine-malachite green solution for _____ before use.

50-60 mg

Approximately _____ of stool, roughly the size of a soybean, is required for a Kato thick smear.

cork stopper

A _____ is used to press the cellophane gently to spread the stool specimen in the Kato technique

30 minutes; 1 hour

Kato slides should be kept at room temperature for _____ to _____ for proper clearing.

causes drying: shells of hookworm ova will dissolve in glycerine.

Why should Kato slides not be allowed to stand for a long period of time?