19 - Pseudomonas

Aquatic habitats and soil

Pseudomonas are most commonly found in where?

P. mallei

This pseudomonas specie is a specialized mammalian parasite

P. pseudomallei and P. aeruginosa

These pseudomonas species are occasional parasite of animals

P. mallei

Pseudomonas are all motile by means of polar flagella except?

Rod

What shape is the genus pseudomonas

Oxidase-positive

Pseudomonas are what enzyme positive?

P. pyocyaneus

This pseudomonas specie is the bacillus of green pus

Rod-shaped

What shape is the genus Pseudomonas?

Chicken

Pseudomonas is common in intestinal contents of __ , and can result in early meat spoilage

P. aeruginosa

Causes “green wool” in sheep, associated with wetting of the fleece

Gram negative

Is pseudomonas Gram + or -

aminoacetophenone

Pseudomonas is recognized by its green pigment, and grape-like odor ___ ?

Fluorescein

This Pseudomonas pigment consists of a pyoverdine that is yellow green, that oxidizes to yellow

Fluorescein

This Pseudomonas pigment is a bluish green pigment that oxidizes to brown

Pycocyanin

This Pseudomonas pigment is a bluish green pigment that oxidizes to brown

Pyocyanin

Fluorescein and pyocyanin are water soluble but only which is soluble in chloroform?

Potassium gluconate medium

Apyocyanogenic strains’ mucoid growth can be identified on what medium?

terminal electron receptor

Pseudomonas are obligate aerobes that uses oxygen as

4 to 42 Celsius

Pseudomona’s range of incubation temp ranges from ___

fimbriate

Pseudomonas produce smooth, shiny, moist, __ and spreading colonies

translucent

Pseudomonas colonies have thin, irregular margins and __ centers are cream colored although often disguised by green pigment

Opalescent sheen

Pseudomonas colonies have what on the surfaces of growths on solid media?

Carbohydrates

Pseudomonas strains mostly do not ferment __

Negative

Pseudomonas are Indole __?

Negative

Pseudomonas are MR __?

Negative

Pseudomonas are VP __?

Positive

Pseudomonas are Catalase and Oxidase __?

O

Pseudomonas have 17 _ antigens

H

Pseudomonas have 6 __ or flagellar antigens

Elastin

Pseudomonas toxins have toxin A, alkaline proteases and elastase which destroys __ in lung parenchyma

Toxin A

This toxin in Pseudomonas inhibits protein synthesis and contributes significantly to lesion development in burn infections

Pyocin

Pseudomonas produces this bacteriocin used in typing, it kills other strains of same species

Skin

Pseudomonas is a component of the normal bacterial flora of __

Soil and water

Pseudomonas’ presence reflects exposure to environment such as __

Toxin A and protease

These are important in Pseudomonas’ edema production, induration, hemorrhage and necrosis observed in skin lesions

P. aeruginosa

This Pseudomonas specie is found in necrotic pneumonias, enteritis and rhinitis in swine, lesions associated with traumatic reticulopericarditis in cattle; bovine mastitis, sporadic abortion (cattle), balanoposthitis (bulls); suppurative otitis externa (dogs)

Minks and chinchillas

P. aeruginosa infection most devastating in __, causing hemorrhagic pneumonia; organism enters when animal sniffs food

Green wool or fleece rot

This results from growth of the organism in fleece subjected to prolonged wetting favored by protein that leaks out of macerated skin; dermatitis results and wool fibers separate; protein degrades resulting in odor that stimulates oviposition by flies predisposing to development of strike