Activity 5 | Paper Chromatography

Chromatography

A technique that is used to separate mixtures into their constituent components

Spotting

Applying the sample to the paper

Developing chamber

A container that contains 1-2 cm of a developing solvent in the bottom

Stationary phase

The chromatography paper

Mobile phase

The solvent traveling up the paper during the separation

Solvent front

The final location of the solvent after it travels up the paper

Retention factor (R_f)

= distance traveled by substance (cm) / distance traveled by solvent front (cm)

Developing

The process of separating the components of each spot

Chromatogram

The paper with the separated substances at the end of developing

The developing chamber is covered with plastic wrap or foil so that the developing solvent and its vapors come to _____

equilibrium

Co²⁺ color in KSCN

Light blue

Cu²⁺ color in KSCN

Black

Fe³⁺ color in KSCN

Blood red

Ni²⁺ color in K₄Fe(CN)₆

Light blue-green