Serology (SNAP Testing and In-House Diagnostics)

The study of detection and measurement of antibodies and antigens

Serology

very specific antibodies that are commercially produced for use in serology test kits to detect the prosence of disease

Monoclonal antibodies

3 Main types of Serology Testing

Latex Agglutination, Enzyme Linked Immunosorbent Assay (ELISA), Rapid Immunomigration (RIM)

Suspension of small, latex particles that have been coated with antibody (sometimes antigen) is added to the serum sample, If corresponding antigen is present, it will react with antibody, visible clumping will appear

Latex Agglutination

Most common test used, Detects presence of antient,

ELISA

Serum Chemistry Evaluation evaluates

organ function and electrolytes

Organs normally tested on Serum Chemistry Evaluation

Liver, Kidneys, Pancreas, Gallbladder (not in Horses, G Pigs, Rabbits)

Serum Glucose Test tests

blood sugar

Instead of an enzyme reaction or latex particles clumping, this is an accumulation of colloidal gold that causes color change

RIM

Other name for RIM

Lateral Flow Assays

What part of blood is used for testing clotting factors

plasma

Complete Blood Count Blood/vacutainer used

Whole blood, purple top

Measures the time that it takes for a fibrin clot to form after calcium and an activator are added to a blood sample

PTT

PTT

Activated partial thromboplastin time

What is added to PTT blood sample to form fibrin clot

calcium and an activator

Measures the amount of time it takes for a fibrin clot to form after calcium and a tissue-activating factor are added to a blood sample

PT

PT

Prothrombin time

What is added to blood sample of PT to form fibrin clot

calcium and a tissue-activating factor

measures the conversion of fibrinogen to a fibrin clot

TT

TT

thrombin time