Campylobacter

What is the shape of Campylobacter

S shape

What kind of flagella does campylobacter posses?

single polar flagellum

Does campylobacter use carbohydrates?

No

Non-pathogenic Campylobacter spp. Found on __________ of the ________and intestinal tracts

mucous membranes, genital, and intestinal tracts

What are the two subsp of C. fetus

C. fetus (subsp. Fetus and subsp. venerealis)

Cause of bovine genital campylobacteriosis

C. fetus subsp. Fetus

C. fetus subsp. venerealis

C. jejuni

C. fetus subsp. venerealis

C. fetus subsp. venerealis found in __________ of asymptomatic bull and

___________ of cow and heifer

preputial cavity, genital tract

Mode of infection?

semen

C. fetus subsp. fetus occurs in __________ of cattle and sheep and in the __________ of infected sheep and cattle

intestines and genital tract

causes sporadic abortion in cattle and multiple abortions in sheep

C. fetus subsp. fetus

Direct examination demonstration of the organism in the ________contents

by negative staining and by phase or _____________

fetal stomach and dark field microscopy

used to identify C. fetus in preputial washings, cervical mucus and fetal stomach contents

FAT

used to detect subsp venerealis in semen

PCR

What is the Specific immunity involves in this disease?

• intestinal IgA (skin and mucous membrane)

• systemic antibodies IgM

• IgG

Blood agar should incubate at ?

37'C and 10% CO2, 5% 02, 85% N2

What is the surface protein of C. fetus

99-kDa protein

Id of Campylobacter spp. Isolates based on___________gene sequences

16S ribosomal RNA

What test can we used Cervical and vaginal mucus and moderately reliable

Agglutination test

Irrigation of uterus and prepuce with____________ to washout or

flash-out the organism

streptomycin

True or false

Semen for Al treated C. fetus subsp. Venerealis can be eliminated from herd but not

subsp. Fetus

True

Which subspecies can cause human infections

C. fetus subsp fetus

Occurs as commensal of intestinal tracts of domestic and wild animals including

birds, poultry, dogs and cats and sometimes it can cause disease if lower immunity

C, jejuni

What are the virulence factor of C. jejuni? (ACEE)

Adhesin, cytotoxin, enterotoxin,endotoxin

What toxin is present C. jejuni which is similar to cholera toxin, activates adenylate

cyclase

Enterotoxin

What will happen if there is increases levels of cyclic AMP?

Diarrhea

Toxin of C. jejuni that causes local tissue destruction and abscess formation

Cytotoxin

Cause of avian infectious hepatitis in chickens and turkeys; hemorrhagic and necrotic changes in the liver

C. jejuni

causes infections characterized by diarrhea and enterocolitis in dogs, cats, sheep, gots, calves, minks, ferrets and other animals

C. jejuni

cause stomach cancer and similar to ulceration

H.pylori

What is the protective immunity for H.pylori

No protective immunity

H. pylori is transmission is via ?

feco-oral or oral-oral

H. pylori breakdown _______ producing ammonia and CO2 thus raising pH,

urea

most common species of helicobacter in dogs and cats

H. heilmannii