Chemistry - Chromatography

Chromatography

a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively absorbent stationary phase

Principle of Chromatography

relative attraction of components of mixture for mobile phase compared to stationary phase

Mobile Phase

water and alcohol

Stationary Phase

paper

Gas

mobile phase in gas chromatography

Non Volatile Liquid

stationary phase in gas chromatography

Uses of Gas Chromatography

• drug tests on athletes

• blood alcohol tests

High Performance Liquid Chromatography

HPLC

Uses of HPLC

• examining growth promoters in meat

• examining vitamins in foods

Principle of Gas Chromatography

a gaseous mobile phase is in contact with a stationary phase and separation of the mixture occurs as a result of selective absorbance of the components of the mixture on the stationary phase

Principle of HPLC

liquid mobile phase is in contact with a stationary phase (fine particles of a solid) and separation of the mixture occurs as a result of selective absorbance of the components of the mixture on the stationary phase