240E1 molecular techniques

in situ hybridization

mRNa where within tissue is mRNA expressed?

immunohistochemistry / immunofluorescence

protein ; where within tissue is protein expressed?

western blot

protein is protein expressed by the cell? semi quantitative

PCR

DNA ; is a specific sequence of DNA present? is the gene still expressed?

DNA sequencing

DNA

what is the nucleotide sequence of a specific DNA?

RT-PCR

mRNA ; is mRNA expressed?

qRT-PCR

mRNA ; how much mRNA is xpressed by a cell or tissue?

indirect ELISA

antibody; quanitfy the amount of antibody in a patient sample

direct ELISA

protein ; quantify the amount of protein in a patient sample (fluid)

Lateral flow ELISA

protein ; is protein present in a patient sample?

flow cytometry

DNA; what stage of the cell cycle is a cell in? How much DNA is found in the cell?

reporter gene assays

DNA ; where within a tissue are the promoter or regulator regions of a gene active? what cells the protein is expressed in.

transgene assay

DNA; where in the cell is a protein expressed?