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light microscope
an optical instrument with lenses that refract visible light to magnify images of specimens
electron microscope
a microscope that focuses an electron beam through a specimen, resulting great resolving power
cell fractionation
the disruption of a cell and separation of its organelles by homogenization and centrifugation
organelle
structure found within a cell that performs a specific task
centrifugation
a technique that uses spinning force to separate particles from a suspension
pellet
fraction of cell components that settles at the bottom of a tube after centrifugation
supernatant
liquid that collects above the pellet in a tube after centrifugation
scanning electron microscope (SEM)
a microscope that uses electrons to show the surface of a sample, useful for studying topography of a specimen
transmission electron microscope (TEM)
a microscope that uses electrons to study a thin specimen, used to study ultrastructure of cells
eukaryotic cell
cell that contains a nucleus and other structures (organelles) enclosed within membranes
prokaryotic cell
a cell that has no membrane bound organelles
cytosol
the semifluid portion of the cytoplasm
nucleoid
a dense region of DNA that is found in a prokaryotic cell
cytoplasm
the entire contents of the cell, exclusive of the nucleus, and bounded by the plasma membrane
plasma membrane
the membrane at the boundary of every cell that acts as a selective barrier
nuclear envelope
the membrane structure in eukaryotes that encloses the nucleus, separating it from the cytoplasm
nuclear lamina
a netlike array of protein filaments that maintains the shape of the nucleus
chromosome
a threadline, gene-carrying structure found in the nucleus or nucleoid. each consists of one very long coiled DNA molecule
chromatin
the complex of DNA and proteins that makes up a eukaryotic chromosome when it is not coiled
nucleolus
a specialized structure in the nucleus that is active in the synthesis of ribosomes
endomembrane system
the collection of membranes inside and around a eukaryotic cell, related either through direct physical contact or by the transfer of membranous vesicles
smooth endoplasmic reticulum (smooth ER)
endoplasmic reticulum (ER) that lacks ribosomes
rough endoplasmic reticulum (rough ER)
endoplasmic reticulum (ER) that contains ribosomes, manufactures membranes and secretory proteins
vesicle
a small sac surrounded by a membraine inside of cells
glycoproteins
proteins that have carbs covalently bonded to them
transport vesicle
a tiny membranous sac in a cell’s cytoplasm carrying molecules produced by the cell
golgi apparatus
an organelle that modifies products of the ER, such as proteins and sends them to other destinations in the cell
lysosome
a sac of hydrolytic enzymes that is used to digest macromolecules or cellular debris
phagocytosis
a method used by cells for engulfing large particles by surrounding them with the plasma membrane
autophagy
a process when lysosomes use their hydrolytic enzymes to recycle the cell’s own organic material
vacuoles
large vesicles derived from ER and golgi apparatus, mostly used to store materials or waste
endosymbiotic theory
explanation for how the early ancestor of eukaryotic cells engulfed prokaryotic cells that eventually evolved into the mitochondria and chloroplasts
cristae
infoldings of the inner membrane of a mitochondrion
mitochondrion
organelle that is the site of cellular respiration
mitochondrial matrix
the compartment of the mitochondrion enclosed by the inner membrane
thylakoid
a flattened membrane sac inside the chloroplast, used to convert light energy to chemical energy
granum
a stack of thylakoids found in the chloroplast
stroma
the fluid outside the thylakoid found inside a chloroplast
plastid
members of a family of closely related plant organelles that contain pigments or reserve materials such as starch
photosynthesis
a process used by plants and other organisms to convert light energy, carbon dioxide and water into chemical energy
peroxisome
an organelle containing enzymes that degrade hydrogen peroxide into water and oxygen
microtubule
a hollow rod of tubulin protein in eukaryotic cells that forms part of cilia, flagella, and the cytoskeleton
centrosome
the microtubule organizing center, present in the cytoplasm of all eukaryotic cells, it is involved in cell division
centrioles
structures found in an animal cell composed of cylinders of microtubule triplets. an animal cell usually has a pair of them that are involved in cell division
flagella
long cellular appendages specialized for locomotion
cilia
short cellular appendages specialized for locomotion
basal body
a eukaryotic cell structure made out of microtubule triplets. they are part of cilia and flagella
microfilament
a solid rod of actin protein in the cytoplasm of eukaryotic cells, making up part of the cytoskeleton
actin
a globular protein that links into chains, forming microfilaments in muscle and other contractile elements in cells
myosin
the contractile protein that makes up the thick filaments of muscle fibers
pseudopodia
a cellular extension of cells used in moving and feeling
cytoplasmic streaming
a circular flow of cytoplasm that speeds the distribution of materials within cells
intermediate filament
a component of the cytoskeleton that includes all filaments with size between that of microtubules and microfilaments
primary cell wall
a relatively thin and flexible layer first secreted by a young plant cell
middle lamella
a thin layer of adhesive extracellular material, primarily pectins, found between the primary walls of adjacent young plant cells
secondary cell wall
a layer of the cell wall that is rigid, used for plant cell protection and support in woody plants
extracellular matrix (ECM)
an intricate network of molecules, found outside the cell, that link together to form a stable layer
collagen
a glycoprotein in the ECM of animal cells that forms strong fibers; the most abundant protein in the animal kingdom
cell junctions
structures that provide contact between neighboring cells or a cell and the ECM
tight junctions
structures where plasma membrances of neighboring cells are pressed against each other, forming continuous seals around the cell
desmosomes
anchoring junctions that function like rivets, specialized for cell-cell adhesion
gap junctions
structures that provide cytoplasmic channel connections from one animal cell to an adjacent cell
plasmodesmata
an open channel in the cell wall of a plant through which the cytosol connects to the cytosol from an adjacent cell
amphipathic
a molecule that has both a hydrophilic region and a hydrophobic region
fluid mosaic model
the currently accepted model of cell membrane structure, which envisions the membrane as a mosaic of individually inserted protein molecules drifting laterally in a fluid bilayer of phospholipids
integral proteins
proteins that extend entirely through the membrane with both hydrophobic and hydrophilic parts
peripheral proteins
protein loosely bound to the surface of the membrane and not embedded in the liquid bilayer
transmembrane proteins
a protein that goes from one side of the cell membrane to the other side
glycolipids
lipid substances with linked sugar groups
permeability
a measure of the rate of passive diffusion of molecules through the membrane
transport proteins
protein molecules that help to carry substances throughout the body and across cell membranes
channel proteins
a type of transport protein that functions by having a hydrophilic channel that certain molecules or ions use as a tunnel through the membrane
aquaporins
a transport protein in the plasma membrane of a plant or animal cell that specifically facilitates the diffusion of water across a membrane
diffusion
the process by which molecules spontaneously move from an area of higher concentration to an area of lower concentration
concentration gradient
the difference in the concentrations of a substance across a barrier or membrane
passive transport
the movement of substances with gradient across a cell membrane without the use of energy by the cell
osmosis
diffusion of water molecules through a semipermeable membrane until the concentration on both sides is equal
isotonic
having a solute concentration equal to that of another solution
hypertonic
when comparing two solutions, the solution with the greater concentration of solutes
hypotonic
when comparing two solutions, the solution with the lesser concentration of solutes
turgid
firm, walled cells become firm as a result of the entry of water from a hypotonic environment
flaccid
limp, lacking in stiffness or firmness, as in a plant cell in surroundings where there is no tendency for water to enter the cell
plasmolysis
when a cell is in a hypertonic environment, the cell will lose water to its surroundings, shrink, and its plasma membrane will pull away from the wall
facilitated diffusion
the transport of substances through a cell membrane along a concentration gradient with the aid of transport proteins
ion channels
a transmembrane protein channel that allows a specific ion to flow across the membrane down its concentration gradient
gated channels
a protein channel in a cell membrane that opens or closes in response to a particular stimulus
active transport
the movement of a substance across a biological membrane against its concentration of electrochemical gradient
sodium-potassium pump
a special transport protein in the plasma membrane of animal cells that transports sodium out of the cell and potassium into the cell against their concentration gradient
membrane potential
the voltage difference between a cell’s cytoplasm and the extrcellular fluid, due to the differential distribution of ions
electrochemical gradient
a transport force driven by both a difference in charges and in concentration
electrogenic pump
a transport protein that generates a voltage across a membrane
proton pump
an active transport mechanism in cell membranes that consumes ATP to force hydrogen ions out of a cell
cotransport
the coupling of the “downhill” diffusion of one substance with the “uphill” transport against its own concentration gradient
exocytosis
the process by which a substance is released from the cell through a vesicle
endocytosis
the process by which a cell membrane surrounds a particle and encloses the particle in a vesicle to bring the particle into the cell
pinocytosis
a type of endocytosis in which the cell ingests extracellular fluid and its dissolved solutes
receptor-mediated endocytosis
the movement of specific molecules into a cell by the inward budding of vesicles containing proteins with receptor sites specific to the molecules being taken in
carrier proteins
proteins that bind to molecules and change shape to shuttle them across the membrane