Determination of DNA Concentration and Purity

Determination of DNA Concentration and Purity

It estimates the DNA Concentration from the absorbance of the onion DNA solution and assess the DNA Purity by calculating the A₂₆₀ / A₂₈₀ Ratio

Protein/Phenol

The Determination of DNA Concentration and Purity reflects ________ or RNA Contamination

Nucleotide

Nucleic Acids are long-chain polymers made up of repeating units called ________

Pentose Sugar, Phosphate Group, and Nitrogenous Base

Each Nucleotide consist of a _________ (ribose or deoxyribose), a __________, and a __________ (adenine, guanine, cytosine, thymine, or uracil)

260

Nucleic acids (bases with conjugated double bonds) show maximal UV Absorbance at ________ nm

280

Aromatic Amino Acids in proteins absorb maximally at _______ nm

dsDNA

For ________ in a 1-cm cuvette, A₂₆₀ for absorbance