Indirect Elisa
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Last updated 10:54 AM on 4/25/25
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4 Terms
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Steps
Known antigen absorbed on 96 well plate
Patient serum added
If present: primary bodies bind to antigen
Enzyme linked secondary antibodies added → bind to Fc region of primary antibodies
Rinse plate & bound complex remain.
Substrate added → enzyme reacts → color change.
Look at results with Spectrophotometer
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Testing for
HIV, HCV & HBV
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Purpose
Detects unknown antibodies, IgG or IgM to diagnose viral infections.
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Why sensitive
Multiple secondary ABs can bind to one primary AB which amplifies enxyme signal and improves detection even when antibody concentration is low.
Note 
Flashcards (42)