Indirect Elisa

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4 Terms

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Steps

  1. Known antigen absorbed on 96 well plate

  2. Patient serum added

  3. If present: primary bodies bind to antigen

  4. Enzyme linked secondary antibodies added → bind to Fc region of primary antibodies

  5. Rinse plate & bound complex remain.

  6. Substrate added → enzyme reacts → color change.

  7. Look at results with Spectrophotometer

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Testing for

HIV, HCV & HBV

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Purpose

Detects unknown antibodies, IgG or IgM to diagnose viral infections.

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Why sensitive

Multiple secondary ABs can bind to one primary AB which amplifies enxyme signal and improves detection even when antibody concentration is low.