Early Antibody Approaches and Antisera
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10 Terms
Where are antibodies found after immunisation?
In the plasma of the blood
What is serum?
Plasma with clotting factors removed
What is antiserum?
Serum from an immunised individuals containing antibodies
Why does antiserum contain many antibodies?
Because multiple B cell clones respond to immunisation
What is polyclonal antiserum?
Serum containing more than one antibody specificity
Can different antibodies bind the same antigen?
Yes, they may find different epitopes
Why is antiserum still useful despite being polyclonal?
It contains high concentrations of antigen-specific antibodies
What proteins are removed during antiserum purification?
Other serum proteins such as albumin
How does gel filtration chromatography spoearate proteins?
Based on molecular weight
Why is affinity chromatography superior for antibody purification?
It selectively binds antigen-specific antibodies
Note 
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