chap 6b (microscope)
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46 Terms
Why are organelles not visible to the naked eye?
They are very small and require magnification using a microscope.
What is the maximum magnification of a light microscope?
About 2000×.
What is the maximum magnification of an electron microscope?
About 500 000×.
Where is the eyepiece located?
At the top of the microscope.
What is the function of the eyepiece (ocular)?
Contains lenses to further magnify the image.
Where is the body tube located?
Between the eyepiece and objective lenses.
What is the function of the body tube?
Holds the eyepiece and objective lenses at the correct distance.
Where is the nosepiece located?
Below the body tube.
What is the function of the nosepiece?
Holds objective lenses and allows switching between them.
Where are the objective lenses located?
Attached to the revolving nosepiece.
What is the function of objective lenses?
Provide different magnifications of the specimen.
Which objective lens is the shortest?
The low-power objective lens (4× or 10×).
Which objective lens is the longest?
The high-power objective lens (40× or 60×).
Where is the stage located?
Below the objective lenses.
What is the function of the stage?
Holds the microscope slide.
What are stage clips used for?
To secure the slide in position.
Where is the condenser located?
Below the stage.
What is the function of the condenser?
Focuses light onto the specimen.
Where is the diaphragm located?
Below the stage, near the condenser.
What is the function of the diaphragm?
Controls the amount of light passing through the specimen.
Where is the coarse adjustment knob located?
On the side of the microscope.
What is the function of the coarse adjustment knob?
Focuses image under low power by moving the stage.
Where is the fine adjustment knob located?
On the side, smaller than the coarse knob.
What is the function of the fine adjustment knob?
Sharpens focus under high power.
Where is the light source located?
At the base of the microscope.
What is the function of the light source?
Directs light through the specimen.
Where is the arm located?
On the side connecting the body to the base.
What is the function of the arm?
Used to support and carry the microscope.
Where is the base located?
At the bottom of the microscope.
What is the function of the base?
Provides stability and support.
What is the first objective lens used when viewing a specimen?
The lowest power objective lens (4×).
Why must the objective lens not touch the slide?
It may damage the slide and lens.
Which knob is used first to focus the specimen?
Coarse adjustment knob.
Which knob is used to sharpen the image?
Fine adjustment knob.
Why is the specimen centred before focusing?
To ensure it remains in view when magnification is increased.
Why is the coverslip lowered at an angle?
To prevent air bubbles from forming.
What stain is used for cheek cells?
Iodine solution.
What tool is used to collect cheek cells?
A toothpick.
What liquid is used when preparing elodea cells?
Water.
What is one rule for good cell drawing regarding tools?
Use pencil only.
How should labelling lines be drawn?
With a ruler, horizontal, and not crossing.
Are arrowheads allowed in cell drawings?
No.
Is shading allowed in cell drawings?
No shading or colouring is allowed.
How large should a cell drawing be?
About ¾ of the space provided.
Where should the title of a cell drawing be written?
At the bottom and underlined.
Why must drawings use clear, firm lines?
To ensure clarity and accuracy.