3.5 Module

These flashcards cover key concepts related to protein purification, focusing on metrics like yield and specific activity, as well as methods like centrifugation and chromatography.

Protein Purity

A measure of how much of a target protein has been isolated from a mixture, minimizing the presence of contaminants.

Yield

The total amount of target protein recovered after each purification step, indicating the effectiveness of the purification process.

Specific Activity

A measure that relates the activity of a target protein to its concentration, helping to assess protein purity.

Centrifugation

A technique that exploits differences in size, shape, and density among cellular components to separate proteins from cellular debris.

Differential Centrifugation

A method that separates cellular components based on their differing sedimentation rates during centrifugation.

Density Gradient Centrifugation

A technique that separates proteins based on differences in density, facilitating further purification.

Chromatography

A method used after centrifugation for more refined purification of proteins.

Biochemical Workflow

The series of techniques and analyses utilized in biochemistry to study and manipulate proteins and other biomolecules.