Clin Bacte Module 2 (Strep)

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116 Terms

1

Bile Esculin Test (+) result

Blackening

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2

Viridans Strp is the no 1 cause of?

Subacute bacterial endocarditis

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3

LAP TEST (Leucine Amino Peptidase)

Presumptive test for catalse (-) gram (+) cocci

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Substrate of LAP Test

Leucine-beta-naphthylamide

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Reagent of LAP Test

cinnamaldehyde

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(+) result of LAP Test

Red

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(-) result of LAP Test

no color or slight yellow

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Arrangement of strep

chain

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shape of strep

spherical to ovoid

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10

Motility and special requirement of strep

Non-motile; Capnophilic

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11

Medium of Choice for Strep

SBA

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12

Uses of SBA in strep

  • Isolates

  • Growth

  • Determines hemolysis pattern

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13

Selective Medium for strep

PEA - Phenyl Ethyl Alcohol Agar

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14

Organisms + for Lap Test

Enterococcus, Streptococcus, Pediococcus

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Organisms - for Lap Test

Aerococcus & Leuconostoc

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SMITH & BROWN CLASSIFICATION

  • Alpha hemolytic

  • Beta hemolytic

  • Gamma hemolytic

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17

Can cause incomplete/partial hemolysis on BAP

Alpha hemolytic

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18

What hemolysis on BAP can cause greening of agar?

Alpha hemolytic

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19

What hemolysis on BAP can produce clear zones around the colonies?

Beta hemolytic

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20

Beta hemolytic

Can cause complete hemolysis on BAP

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Gamma hemolytic

Do not cause hemolysis on BAP

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22

Organisms under Alpha Hemolytic

Streptococcus pneumoniae,

Viridans Streptococci – S. mitis and S. mutans

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Organisms under Beta Hemolytic

  • S. pyogenes

  • S. agalactiae

  • S. aureus (staph)

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Organisms under Gamma Hemolytic

  • Group D Non-Enterococci / Enterococci

  • Neisseria

  • Moraxella

  • Staph. epidermidis

  • Staph. saprophyticus

  • Micrococci

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25

CAPSULAR SWELLING TEST NEUFELD QUELLUNG +

S. pneumoniae

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CAPSULAR SWELLING TEST NEUFELD QUELLUNG -

VIRIDANS

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BILE SOLUBILITY TEST -

VIRIDANS

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BILE SOLUBILITY TEST +

S. pneumoniae

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OPTOCHIN DISK TEST sensitive

S. pneumoniae

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OPTOCHIN DISK TEST resistant

VIRIDANS

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31

Which strep is encapsulated?

S. pneumoniae

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Which strep is non-encapsulated?

VIRIDANS

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33

BILE SOLUBILITY TEST BAP is added with

10% sodium desoxycholate

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34

BILE SOLUBILITY TEST BAP (+) results

lysis of colonies

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35

BILE SOLUBILITY TEST BAP (-) results

intact colonies

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36

BILE SOLUBILITY TEST broth is added with

2% sodium desoxycholate

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37

BILE SOLUBILITY TEST broth (+) results

clearing

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38

BILE SOLUBILITY TEST broth (-) results

turbidity, partial clearing

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39

Optochin is also known as?

TAXO P

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40

Chemical name of Optochin is –

Ethylhydrocuprein hydrochloride

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41

Zone of inhibition for sensitive optochin disk test

14mm

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42

Zone of inhibition for resistant optochin disk test

VIRIDANS

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43

Normal flora of nasopharynx and oropharynx (URT)

S. pneumoniae

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44

S. pneumoniae is regarded as # 1 cause of

adult bacterial meningitidis

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45

can cause LOBAR PNEUMONIA and OTITIS MEDIA

S. pneumoniae

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46

S. pneumoniae microscopically appears as

s LANCET or BULLET SHAPED DIPLOCOCCI

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After 24 hrs of incubation (young colonies) colonies of S. pneumoniae may appear

dome shaped →Umbonate

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48

After 48 hrs of incubation (aged colonies) of S. pneumoniae growth may appear

  • flat crater like

  • like a coin with a raised rim

  • Nail head appearing colonies or colonies with checker appearance

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49

S. pneumoniae is also known as

Diplococcus Pneumoniae

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50

Otitis media is a

ear infection

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51

Major Virulence factor of Streptococcus pneumoniae

Capsule

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VIRIDANS Streptococci include:

  • S.mitis

  • S.mutans

  • S.salivarius

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VIRIDANS Streptococci produce?

glucans & dextrans

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glucans & dextrans action

promotes attachment on tooth surfaces

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VIRIDANS Streptococci is regarded as the

Major Throat FLORA

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Beta Hemolytic Streptococci are classified under ?

LANCEFIELD CLASSIFICATION

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LANCEFIELD CLASSIFICATION

most significant in identifying and classifying Beta Hemolytic Streptococci; based on the extraction of C carbohydrate from the Streptococcal cell wall

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58

Streptococcus pyogenes is regarded as the?

Major Throat pathogen

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59

Streptococcus pyogenes is a ______ bacteria

flesh eating

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60

Streptococcus pyogenes can cause

  • Pharyngitis

  • Scarlet fever

  • Necrotizing fascitis

  • Erysipelas

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rapidly progressing skin infection

Necrotizing fascitis

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level up pharyngitis w/ rashes

Scarlet fever

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Erysipelas

skin infection

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Post Sequelae

  1. AGN (Acute Glomerular Nephritis)

  2. Rheumatic Factor

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Identification Tests for Strep Pyogenes

  1. PYR Test

  2. BACITRACIN DISK TEST

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PYR TEST

Pyrrolidonyl – alpha-naphthylamide

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PYR TEST uses

n-n dimethylaminocinnamaldehyde

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PYR TEST (+) result

red

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BACITRACIN DISK TEST for S. pyogenes

Sensitive

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Purpose of PYR Test:

  1. to detect S.pyogenes

  2. to detect enterococci

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Purpose of Bacitracin Disk Test

  1. to detect S.pyogenes

  2. to detect staphylococci from micrococci

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72

What strep is the # cause of Neonatal Meningitis

S. agalactiae

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73

In adult of S. agalactiae

Post partum endometriosis

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Todd Hewitt Broth

selective enrichment media to detect genetal carriage of S. agalactiae during pregnancy

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LIM Broth

enrichment media

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Identification Tests for S.agalactiae

  1. HIPPURATE HYDROLYSIS TEST

  2. CAMP TEST – Christie Atkins Munch Peterson

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The positive result of HIPPURATE HYDROLYSIS TEST is due to the organism’s ability to produce the enzyme

HIPPURATE HYDROLASE / HIPPURICASE

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HIPPURATE HYDROLYSIS TEST media

Sodium Hippurate Broth

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HIPPURATE HYDROLYSIS TEST End products

benzoic acid and glycine

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To detect benzoic acid

ferric chloride (indicator)

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To detect Glycine

ninhydrin reagent (faster)

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CAMP TEST – Christie Atkins Munch Peterson Required media

BAP

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CAMP TEST known organism

S.aureus

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CAMP TEST manner of streaking

Perpendicular

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CAMP TEST (+) result

enhance hemolysis as shown by arrow head zone of beta hemolysis

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CAMP TEST (-) result

No enhanced hemolysis

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Virulence Factors of S. pyogenes

  • M protein

  • Streptolysin O

  • S Non-antigenic

  • Protein F

  • Erythrogenic Toxin or Pyrogenic Toxin

  • Hyaluronidase; Streptokinase

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Prevents phgocytosis

M Protein

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Antigenic (anti-streptolysin O) Oxygen labile, can cause sub-surface hemolysis Streptolysin

Streptolysin O

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Oxygen stable can cause surface hemolysis

Streptolysin S

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promotes attachment to ET cells

Protein F

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cause rashes in scarlet fever

Erythrogenic Toxin or Pyrogenic Toxin

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Hyaluronidase

spreading factor

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Streptokinase

promotes dissolution of clot

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DIAGNOSTIC TESTS for SCARLET FEVER

DICK’S TEST

SCHULTZ-CHARLTON BLANCHE PHENOMENON

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DICK’S TEST

Susceptibility test for scarlet fever

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SCHULTZ-CHARLTON BLANCHE PHENOMENON

test to determine if rashes are due to scarlet fever or not

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DICK’S TEST (+) result

redness at the site of injection

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99

Major Virulence factor of S.pyogenes

M protein

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SCHULTZ-CHARLTON BLANCHE PHENOMENON (+) result

fading of rashes/gradual disappearance of rashes

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