Blood Banking Reagents Review BB 1

Review flashcards covering blood banking reagents, their regulation, testing methods, and quality control procedures.

What are the four basic categories of blood bank reagents?

RBCs with known antigens, antisera with known antibodies, antiglobulin reagents (Anti-IgG or Anti-Compliment), and potentiators to enhance antigen/antibody reactions.

Why must commercial blood bank reagents be licensed by the FDA?

They must meet safety and efficacy standards for use in medical testing and diagnostics.

What information must be provided in the package inserts of blood bank reagents?

Detailed description of the reagent, procedure for use, interpretation of reactions, performance characteristics, limitations, and quality control requirements.

What is the principle behind the ABO typing test?

Direct agglutination of red blood cells with a particular reagent indicates the presence of the corresponding antigen.

What reagents are used in the forward typing for ABO blood type?

Anti-A and Anti-B reagents.

In the ABO/Rh forward typing, what are the expected results for group A?

Agglutination with Anti-A and no agglutination with Anti-B.

What can cause false positive results in ABO tests?

Presence of strong cold autoantibodies and samples drawn into tubes with neutral gel separators.

How are Reverse typing tests performed?

By adding patient plasma to commercial A1 and B red cell reagents.

What does agglutination in the D antigen typing indicate?

The presence of the D antigen.

What is the purpose of a negative reagent control in D antigen typing?

To ensure that a false-positive result has not occurred due to the presence of a panagglutinin.