PARA_Prelims_Lab: AMOEBA SARCODINA (in progress)

2 stages of Protozoan Development

• Cysts

• Trophozoite

Cyst

• Non-motile, Non-Feeding stage, Resistant to acid

• Infective stage

Encystation vs Excystation

• Encystation: Trophozoite → Cyst

• Excystation: Cyst → Trophozoite (exit the cyst stage)

Entamoeba Histolytica (Trophozoite)

Trophozoite

• a stage that is Motile, Feeding stage, Fragile

• Feeds, multiplies and use pseudopods

True

T/F:

• Easily destroyed by gastric juices of the stomach and susceptible in the environment

Trophozoite

(Trophozoite/Cysts) are not usually transmtted to human

Pseudopods

is the extension of the cytoplasm that is used for motility

karyosome

• the chromatin found inside the nucleus

• Things to consider: size, and location

Peripheral chromatin

• This is the chromatin that surrounds the nuclear memrbane

• Things to consider: distribution (even or uneven)

Entamoeba histolytica (cyst)

Life Cycle of Entamoeba histolytica

Clinical Symptoms fro E. histolytica

• Symptomatic Intestinal Amebiasis

• Symptomatic Extraintestinal Amebiasis

Entamoeba coli (Trophozoite)

What form is the pseudopods for Entamoeba coli

• blunt pseudopod

Blunt pseudopods

• this type of pseudopod is sluggish and nonprogressive

• broad and short

Finger-like pseudopods

• this type of pseudopods is rapid and progressive

• narrow and long

Entamoeba coli (Cyst)

Entamoeba coli (trophozoite)

Motility
No. of nuclei
Cytoplasmic inclusion

Motility	Blunt pseudopod
No. of nuclei	one
Cytoplasmic inclusion	Vacuoles containing bacteria

Entamoeba coli (Cyst)

Motility
No. of nuclei
Cytoplasmic inclusion

Motility	Blunt pseudopodia
No. of nuclei	1-8
Cytoplasmic inclusion	pointed to splintered ends Chromatoid bars

Entamoeba hartmanii (trophozoite)

Motility
No. of nuclei
Cytoplasmic inclusion

Motility	Finger-like pseudopod
No. of nuclei	One
Cytoplasmic inclusion	Ingested bacteria may be present

Entamoeba hartmanii (Cyst)

Motility
No. of nuclei
Cytoplasmic inclusion

Motility	Spherical
No. of nuclei	1-4
Cytoplasmic inclusion	Chromatoidal bars, rounded ends in young cysts Diffuse glycogen mass

Endolimax nana (trophozoite)

Motility
No. of nuclei
Karyosome
Cytoplasmic inclusion

Motility	blunt pseudopods
No. of nuclei	One
Karyosome	Large, irregular, blotlike
Cytoplasmic inclusion	Bacteria

Endolimax nana (Cyst)

Motility
No. of nuclei
Karyosome
Cytoplasmic inclusion

Motility	Spherical, ovoid, ellipsoid
No. of nuclei	1-4
Karyosome	Large, blotlike, usually central
Cytoplasmic inclusion	Chromatin granules nondescript small mass Diffuse glycogen mass in young cyst

Shape of Karyosome in Endolimax nana (trophozoite)

Blot-like karyosome

Iodameoba butschlii (trophozoite)

Motility
No. of nuclei
Cytoplasmic inclusion
Peripheral Chromatin

Motility	Sluggish, usually progressive (blunt)
No. of nuclei	one
Cytoplasmic inclusion	Bacteria Yeast Other debris
Peripheral Chromatin	Absent

Iodameoba butschlii (cyst)

Motility
No. of nuclei
Peripheral Chromatin
Cytoplasmic inclusion

Motility	Ovoid, ellipsoid, triangular
No. of nuclei	One
Peripheral Chromatin	Absent
Cytoplasmic inclusion	Well-defined glycogen mass Granules may be present

Balantidium coli (Trophozoite)

Size Range
Motility
No. of nuclei	macronucleus: micronucleus:

Size Range	28-152 um in length 22-123 um wide
Motility	Rotary, Boring
No. of nuclei	Kidney shaped (macronucleus) small spherical (micronucleus)

Balantidium coli (Cyst)

Size Range	—
Number and apparatus of nucleus	macronucleus micronucleus
Other features

Size Range	—
Number and apparatus of nucleus	macronucleus: Two Kidney shaped micronucleus: Small spherical
Other features	One or two visible contractile vacuoles Double cyst wall Row of cilia visible between the two cyst wall layers