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what is the agonist for muscle contraction
acetylcholine
what is the antagonist for muscle contraction
atropine
7 pieces of equipment used
organ bath
100ml krebs reservior
heated water bath
force tranducer
chart recorder
agonist syringe
antagonist syringe
function of the organ bath + what is it comprised of
allows living tissue to be exposed to different conditions without cells being damaged
krebs solution: contains glucose & has a similar ionic composition to tissue fluid
function of 100ml krebs reservoir
contains large volumes of krebs solution so the organ bath can be replaced quickly
why must the organ bath be replaced with new krebs solution quickly
so drug added to the organ bath can be washed out & stops tissue from drying out
why is glucose added to the krebs solution
to supply the energy requirements of the tissue
why is the krebs solution buffered with Tris
so the pH is in the physiological range
muscle contractions in the ileum are very sensitive to pH change
mimics conditions in the lower abdomen
function of the heated water bath
37C (body temperature)
warm water circulates around tissue to maintain the correct temperature (so that enzyme/ion channel activity isn’t affected)
mimics in vivo conditions
allows metabolic reactions to happen at their normal rate
function of the force transducer
detects contraction of muscle tissue & converts it to what we see on the chart recorder
attaches to the upper end of the tissue
function of the chart recorder
visual representation of the contraction/relaxation of muscle tissue
what is EC50
the concentration of a drug which causes a response halfway between the baseline and maximum
how to calculate EC50
look on concentration curve for 50% of maximum response
axis of a concentration curve
x: log [agonist]
y: muscle contraction (g)
what statistical test would you do to see if antagonist effects the agonist for muscle contraction
2 sample paired t-test
what receptor is activated in the smooth muscle of the gut during contraction
muscarinic acetylcholine receptor