practical 4- guinea pig ileum simulation: concentration response

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16 Terms

1
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what is the agonist for muscle contraction

acetylcholine

2
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what is the antagonist for muscle contraction

atropine

3
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7 pieces of equipment used

  • organ bath

  • 100ml krebs reservior

  • heated water bath

  • force tranducer

  • chart recorder

  • agonist syringe

  • antagonist syringe

4
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function of the organ bath + what is it comprised of

  • allows living tissue to be exposed to different conditions without cells being damaged

  • krebs solution: contains glucose & has a similar ionic composition to tissue fluid

5
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function of 100ml krebs reservoir

contains large volumes of krebs solution so the organ bath can be replaced quickly

6
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why must the organ bath be replaced with new krebs solution quickly

so drug added to the organ bath can be washed out & stops tissue from drying out

7
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why is glucose added to the krebs solution

to supply the energy requirements of the tissue

8
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why is the krebs solution buffered with Tris

  • so the pH is in the physiological range

  • muscle contractions in the ileum are very sensitive to pH change

  • mimics conditions in the lower abdomen

9
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function of the heated water bath

  • 37C (body temperature)

  • warm water circulates around tissue to maintain the correct temperature (so that enzyme/ion channel activity isn’t affected)

  • mimics in vivo conditions

  • allows metabolic reactions to happen at their normal rate

10
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function of the force transducer

  • detects contraction of muscle tissue & converts it to what we see on the chart recorder

  • attaches to the upper end of the tissue

11
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function of the chart recorder

visual representation of the contraction/relaxation of muscle tissue

12
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what is EC50

the concentration of a drug which causes a response halfway between the baseline and maximum

13
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how to calculate EC50

look on concentration curve for 50% of maximum response

14
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axis of a concentration curve

x: log [agonist]

y: muscle contraction (g)

15
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what statistical test would you do to see if antagonist effects the agonist for muscle contraction

2 sample paired t-test

16
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what receptor is activated in the smooth muscle of the gut during contraction

muscarinic acetylcholine receptor