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Paraffin
Rapid method
Paraffin
Cut with ease without distortion
Paraffin
Many stainings are permitted
Paraffin
Prolonged exposure may cause excessive shrinkage and brittleness
Paraffin
Not recommend to fatty tissues
Celloidin
Large and sense specimen
Celloidin
Hard and dense tissue
Celloidin
Neurologic tissue
Celloidin
Less shrinkage; it does not require heat
Celloidin
Cutting thick tissues
Celloidin
Very slow and volatile
Celloidin
Thin sections are difficult to cut
Wet celloidin
It uses 70%-80% alcohol to avoid dehydration and shrinkage
Dry celloidin
Uses Gilsons mixture to make them transparent
LVN
Low viscosity > high concentration> fast penetration
LVN
Cuts thin sectiona due to hard block
Plasticizers
In LVN, this are added to prevent crackings
Gelatin
For delicate and frozen tissue
Gelatin
Water soluble (no dehydration and clearing needed)
Gelatin
Prevents fragmentation of tough and friable tissues when frozen