Biology Exam 3 Review

The initial entry of a protein into the ER involves the use of…

A translocator complex

True or False: ATP is used during the initial transport of a matrix protein across the mitochondrial INNER membrane

False

True or False: Electron microscopes permitted the imaging of subcellular organelles, due to the smaller wavelengths of electron beams vs visible light

True

True or False: The SRP (Signal Recognition Particle) contains both protein and RNA

True.

When the SRP releases the ribosome after ER docking it will be…

In a GDP-bound state

The lipidation of a protein should increase its affinity for…

membranes bilayers

Aggregation in cells is a problem that can be caused by…

unfolded or misfolded proteins

True or False: Oligosaccharyl transferase attaches complex sugar groups containing glucose and mannose (among others) to Asn amino acids in an ER protein

True

If the mannosidase enzyme in the ER quality control machinery was the fastest enzyme (faster than glucosyl transferase or the export machinery) then…

More proteins than normal would be degraded

True or False: If a cell detects that it has too many unfolded proteins, it should want to increase translation

False 

The force that deforms membrane into vesicle-like formation comes from…

Coat proteins like clatherin

Which activity first localizes a vesicle to its target organelle?

Tethering protein function

True or False: Viruses (like flu) use snare-like activity to enable infection

True

A GENETIC approach to identifying a signal sequence would…

Involve the random mutagenesis of genes to identify proteins implicated in protein localization

In addition to proteins, _________________ are synthesized in the ER…

Lipids

True or False: Smooth ER is composed of shorter tubules and contains detoxifying enzymes

True

Transmembrane proteins in the ER are created…

 

Through the sideways gating (into the membrane) by the ER translocation machinery

True or False: Mannosidase is the enzyme in the ER quality control machinery that directly detects if a protein has folded or not

False 

True or False: Exocytosis is the bringing in of plasma membrane and extracellular fluids

False

Which of the following is NOT true of collagen function?

A. It was often defective in pirates

B. It requires the addition of a lipid group to collagen

C. It stabilizes joints

D. It requires protein modifications in the ER

B. It requires the addition of a lipid group to a collagen