pseudosubstrate containing protein that blocks the binding of mTORC1 substrates to Raptor
PRAS40
substrate guiding subunit of mTORC1
Raptor
eIF4E binds most directly to what part of the mRNA?
7-methyl guanosine 5'-5' Cap
methylation of the 5'-5' modification of mRNAs is provided by what substate:
SAM
eIF4G binds to
PABP
PABP binds directly to:
3'A's of the mRNA
ATP-dependent helicase required to destructure mRNAs to allow scanning by the 40S ribosomal subunit
eIF4A
mTORC1 substrate that in its dephosphorylated state interferes with formation of the platform required for eukaryotic translational initiation:
4EBP
4EBP most directly interacts with the part of eIF4E that binds to
eIF4G
recognition sequence on substrates that interact with Raptor:
TOS
most commonly used experimental substrate utilized to measure mTORC1 action:
S6K-T389
bacterially-synthesized compound that specifically inhibits Raptor function:
sirulimus
protein subunit that is bound by Rapamycin which participates in inhibition of mTORC1 function:
FKBP12
substance that binds to the same protein as Rapamycin, but which directs it to inhibit calcineurin
tacrolimus
pentameric complex of protein subunits maintained at the lysosomal membrane cytoplasmic surface by p18's prenylated tails
Ragulator
heterodimer pair that Raptor interacts with to guide mTORC1 to the lysosomal membrane:
Rag A/C
acts as a GAP on RagA:
GATOR1
acts as a GEF on RagC:
Rag A/GTP
inhibitor of the RagA GAP complex:
GATOR2
protein inhibitor of GATOR2'S translation-promoting function that is released by leucine:
sestrin
protein inhibitor of GATOR2's translation promoting function that is released by arginine:
CASTOR
protein inhibitor of GATOR2's translation promoting function that is released by a metabolite of methionine:
SAMTOR
required ligand of RagA in the mTORC1 activating complex:
GTP
portion of the mTORC1 activation complex that specifically bonds to RagC/GDP:
Raptor Claw
prenylated G protein required to complete activation of mTORC1:
Rheb
GAP for Rheb
TSC1/TSC2
modifies the Rheb GAP to tag it for removal from the lysosomal surface:
Akt/PKB
protein that removes the Rheb GAP from the lysosomal surface to the cytoplasm
14-3-3