mTORC1 Practice Quiz

pseudosubstrate containing protein that blocks the binding of mTORC1 substrates to Raptor

PRAS40

substrate guiding subunit of mTORC1

Raptor

eIF4E binds most directly to what part of the mRNA?

7-methyl guanosine 5'-5' Cap

methylation of the 5'-5' modification of mRNAs is provided by what substate:

SAM

eIF4G binds to

PABP

PABP binds directly to:

3'A's of the mRNA

ATP-dependent helicase required to destructure mRNAs to allow scanning by the 40S ribosomal subunit

eIF4A

mTORC1 substrate that in its dephosphorylated state interferes with formation of the platform required for eukaryotic translational initiation:

4EBP

4EBP most directly interacts with the part of eIF4E that binds to

eIF4G

recognition sequence on substrates that interact with Raptor:

TOS

most commonly used experimental substrate utilized to measure mTORC1 action:

S6K-T389

bacterially-synthesized compound that specifically inhibits Raptor function:

sirulimus

protein subunit that is bound by Rapamycin which participates in inhibition of mTORC1 function:

FKBP12

substance that binds to the same protein as Rapamycin, but which directs it to inhibit calcineurin

tacrolimus

pentameric complex of protein subunits maintained at the lysosomal membrane cytoplasmic surface by p18's prenylated tails

Ragulator

heterodimer pair that Raptor interacts with to guide mTORC1 to the lysosomal membrane:

Rag A/C

acts as a GAP on RagA:

GATOR1

acts as a GEF on RagC:

Rag A/GTP

inhibitor of the RagA GAP complex:

GATOR2

protein inhibitor of GATOR2'S translation-promoting function that is released by leucine:

sestrin

protein inhibitor of GATOR2's translation promoting function that is released by arginine:

CASTOR

protein inhibitor of GATOR2's translation promoting function that is released by a metabolite of methionine:

SAMTOR

required ligand of RagA in the mTORC1 activating complex:

GTP

portion of the mTORC1 activation complex that specifically bonds to RagC/GDP:

Raptor Claw

prenylated G protein required to complete activation of mTORC1:

Rheb

GAP for Rheb

TSC1/TSC2

modifies the Rheb GAP to tag it for removal from the lysosomal surface:

Akt/PKB

protein that removes the Rheb GAP from the lysosomal surface to the cytoplasm

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