Phases of Mitosis
prophase, metaphase, anaphase, telophase
Centrioles
Cell organelle that aids in cell division in animal cells only
Prophase
Chromatin condenses into chromosomes and Nucleolus disappears.
Metaphase
chromosomes line up along metaphase plate
Anaphase
Chromosomes break at centrosomes and sister chromatids move to opposite ends of the cell
Telophase and Cytokinesis
Nuclear membrane reforms and nucleoli reappear and chromosomes unwind into chromatin.
What cells do not preform mitosis
Sex Cells
Which cell splits from the inside
Plant cell
telophase under microscope
Which cell forms a cleavage and splits from the outside
Animal cell
anaphase under microscope
metaphase under microscope
prophase under microscope
Base parring
(A-T C-G) This guarantees that a copied strand of DNA always end up being the same as the original.
DNA structure
2 deoxyribose (sugar) backbone strands
Bases held together by \n hydrogen bonds
DNA shape is a double helix
How is DNA fit in a Chromosome
DNA wraps around a Histone to from a Nucleosome
Nucleosomes come together to form Chromatine
Chromatine then from loops
The Chromatine loops condense to form a Chromosome
Helicase
“Unzips” or unwinds the DNA double helix.
Binds at the location called the replication fork
Exposes the leading strand to the next enzyme
Separates bases from each other
DNA Polymerase
Synthesizes free nucleotides onto the template DNA
Matches the right nucleotide with the exposed bases
Creates the complementary strand
DNA Ligase
An enzyme that puts things together
Glues together Okazaki fragments after matching
Primase
Enzyme that makes a primer
The primer is the starting point for polymerase on the lagging strand
Leading strand
replicated (copied) in 3’-5’ direction
copied straight off, no fragments made.
Lagging strand
replicated in 5’-3’ direction
replicated in small pieces, known as Okazaki fragments, starting at a primer
The polymerase has to jump back and make a small section
Ligase glues the Okazaki fragments together
Prophase I
The DNA is already replicated forming two pairs of chromosomes
The homologous sister chromatids pair up 2 and 2
Chromosomes form tetrad
Sometimes parts of the chromosomes are exchanged between the maternal and paternal chromosomes: crossing over or recombination
In meiosis
tetrad
two homologous chromosomes that have each already replicated into a pair of sister chromatids
Metaphase I
The chromosomes are moved to the metaphase plate (the middle) they line up randomly
In meiosis
Anaphase I
The paired homologous chromosomes start to separate from each other
The whole sister chromatid is being moved to either pole
Pulled apart by the mitotic spindle
In meiosis
Telophase I
Cell starts cytokinesis, dividing the cells completely
The cells are now haploid
In meiosis
Haploid
having a single set of unpaired chromosomes.
Diploid
containing two complete sets of chromosomes, one from each parent
Prophase II
Very short phase
Centrioles start migrating
Nucleolus dissolves
Mitotic spindle forms
In meiosis
Metaphase II
Sister chromatids line up at metaphase plate
In a line this time
Spindle fibers attach to the centromere (middle of chromatid)
In meiosis
Anaphase II
Sister chromatids are being pulled to opposite sides of the cell
Sister chromatids break down the middle
Chromatids become separate chromosomes
In meiosis
Telophase II
The chromosomes have completely moved to their cells
Membrane forms around each set of chromosomes
The cells bud off to become individual haploid cells
In meiosis
cytokinesis
bringing about the separation into two daughter cells