Infiltration, Embedding, Microtomy

Impregnation or Infiltration

Refers to the removal of clearing agents

55 - 60 C _{^{[routine: 56 C]}}

Paraffin wax inpregnation TEMPERATURE

• Hard Tissue = Wax with HIGHER melting point

• Soft Tissue = Wax with LOWER melting point

• Hard Tissue = Wax with _____ melting point

• Soft Tissue = Wax with _____ melting point

15 minutes (Wax 1 to 4)

Manual Impregnation:

• Paraffin Wax 1 to 4

• How many minutes?

• Paraplast

• _^Embeddol

• _^Bioloid

• _{^{Ester Wax}}

• _^Carbowax

SUBSTITUTE TO PARAFFIN WAX:

• More elastic and resilient than paraffn

• _^Paraplast

• Embeddol

• _^Bioloid

• _{^{Ester Wax}}

• _^Carbowax

SUBSTITUTE TO PARAFFIN WAX:

• Synthetic and less brittle

• _^Paraplast

• _^Embeddol

• Bioloid

• _{^{Ester Wax}}

• _^Carbowax

SUBSTITUTE TO PARAFFIN WAX:

• Semisynthetic, used for eyes

• _^Paraplast

• _^Embeddol

• _^Bioloid

• Ester Wax

• _^Carbowax

SUBSTITUTE TO PARAFFIN WAX:

• Harder than paraffin

• Soluble in ethyl alcohol

• _^Paraplast

• _^Embeddol

• _^Bioloid

• _{^{Ester Wax}}

• Carbowax

SUBSTITUTE TO PARAFFIN WAX:

• Soluble to water

3 hours

Embedding duration?

• Rocking / Cambridge

• _^Rotary

• _^Sliding

• _^Freezing

• _^Cryostat

• _^Ultrathin

TYPES OF MICROTOMY:

• Cutting LARGE BLOCKS

• Trefall

• SIMPLEST

• _{^{Rocking / Cambridge}}

• Rotary

• _^Sliding

• _^Freezing

• _^Cryostat

• _^Ultrathin

TYPES OF MICROTOMY:

• Most common used

• Minot

• _{^{Rocking / Cambridge}}

• _^Rotary

• Sliding

• _^Freezing

• _^Cryostat

• _^Ultrathin

TYPES OF MICROTOMY:

• For Celloidin Embedded Sections

• Adams

• MOST DANGEROUS!!

• _{^{Rocking / Cambridge}}

• _^Rotary

• _^Sliding

• Freezing

• _^Cryostat

• _^Ultrathin

TYPES OF MICROTOMY:

• for unembedded frozen sections

• QUECKETT

• Profile A: Planoconcave

• _{^{Profile B: Biconcave}}

• _{^{Profile C: Plane Wedge}}

• _{^{Profile D : Chisel Shaped}}

MICROTOME KNIVES:

• 25 mm

• One side is flat

• FOR CELLOIDIN EMBEDDED

• _{^{Profile A: Planoconcave}}

• Profile B: Biconcave

• _{^{Profile C: Plane Wedge}}

• _{^{Profile D : Chisel Shaped}}

MICROTOME KNIVES:

• 120 mm

• Both Side Concave

• for PARAFFIN EMBEDDED

• _{^{Profile A: Planoconcave}}

• _{^{Profile B: Biconcave}}

• Profile C: Plane Wedge

• _{^{Profile D : Chisel Shaped}}

MICROTOME KNIVES:

• 100 mm

• Both side STRAIGHT

• for FROZEN SECTIONS

• for EXTREMELY HARD PARAFFIN

Honing

HONING or STROPPING:

• remove irregularities (nicks)

Honing

HONING or STROPPING:

• Heel to Toe

Stropping

HONING or STROPPING:

• Shapens and Polising (remove burrs)

Stropping

HONING or STROPPING:

• Toe to Heel

• Bevel Angle = 27-32

• Wedge Angle = 15

• Clearance Angle = 5-10

• Bevel Angle =

• Wedge Angle =

• Clearance Angle =

• Water bath set at 45-50*C, then approximately 6-10*C lower than melting point

WATER BATH:

• The water bath is set at what temperature?

• _____ *C lower than the melting point

• Belgium Yellow

• _^Arkansas

• _{^{Fine Carbrundun}}

TYPES OF HONES:

• For manual sharpening

• For nicked and blunted cutting edges

• _{^{Belgium Yellow}}

• Arkansas

• _{^{Fine Carbrundun}}

TYPES OF HONES:

• Gives more polishing effect than Belgium yellow

• _{^{Belgium Yellow}}

• _^Arkansas

• Fine Carbrundun

TYPES OF HONES:

• Much coarser

• used for VERY BAD NICKED KNIVES