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Wright-Giemsa stain properties and steps
eosin - acid stain positive charges vs. methylene blue and Azure B (purple) - basic stain negative charges ]→ methanol dip → stain flood → add buffer → distilled water wash
PBF: Rouleaux
5-4 RBC stacking in monolayer ]- proteins on RBC stick together
PBF: EDTA Satellitism
Platelet clumping due to EDTA ]→ indicates a poor quality film or a rare occurance
Reference Value time
Go to Miro…
PBF Examination steps
Miro (wip)