LAB PRACTICAL 1

Where are the fire extinguisher, fire blanket, and first aid kit kept in the lab?

Under the professor’s desk.

What solution is used to clean the lab bench at the beginning and end of each lab class?

70% ethanol.

Where do wet mounts go for disposal?

In the bleach bath.

What is a colony in microbiology?

A mound of cells found on a petri dish.

How many bacterial cells are needed to start a colony?

One bacterial cell.

What temperature are Petri dishes incubated at for optimal growth?

37°C.

What is the purpose of aseptic technique?

To avoid contamination.

What dye is used in simple staining?

Methylene blue.

Which microscope part collects and directs light?

Condenser.

What is the charge on bacterial cells?

Negative.

What is the primary dye used in Gram staining?

Crystal violet.

What area of the bacterial cell contains the chromosome?

Nucleoid region.

What molecule is targeted in the acid-fast stain?

Mycolic acid.

What does an endospore positive specimen look like under a microscope?

Pink rods and green ovals.

What is the tool used for the stab tube motility test?

Inoculating needle.

What is the movement associated with nonmotile species in a wet mount?

Brownian motion.

Which lens is first used when focusing on a specimen under a microscope?

Scanning lens.

What will happen if a Gram-positive cell is over-decolorized?

It may appear Gram-negative (pink).

What structures are found in the spore stain?

Pink vegetative cells and green endospores.