Prequiz 6

What is the summary reaction for photosynthesis?

6 CO2 + 6 H2O —> C6H12O6 + O2

Where does photosynthesis occur?

chloroplast

What are the two phases of photosynthesis?

Light reactions (in thylakoids) and CO2 fixation (in stroma)

How will you measure the rate of photosynthesis? In other words, what are you measuring/recording?

Measuring changes in the amount of CO2 and O2 surrounding leaves

Name one sample that will be used to measure photosynthesis.

turgid spinach leaves

What is chromatography? Define sample, stationary phase, and mobile phase.

1. Chromatography refers to a set of techniques whereby the components of a mixture can be separated, primarily based on the different solubilities of the mixture components

2. Sample: solubilized in an appropriate solvent, which then moves along or through an inert stationary phase

3. Mobile phase: the dissolved components are separated, and the most soluble travel farthest

4. Stationary phase: the cellulose fibers are compressed into a sheet to form paper

What pigments are of direct importance for photosynthesis?

plastid pigments

How will you transfer plant tissue to the chromatography paper?

Running a coin along the ruler to transfer the plant tissue to the paper on the pencil line

How will you calculate the Rf value for a pigment?

Distance pigment traveled/distance solvent traveled