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1
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In which part of the nephron does maximum reabsorption of glomerular filtrate occur?
A) Loop of Henle
B) Collecting duct
C) Distal convoluted tubule
D) Proximal convoluted tubule

The correct answer is D) Proximal convoluted tubule.

🔵 Explanation:

  • Maximum reabsorption (about 70-80%) of water, glucose, amino acids, and salts happens in the proximal convoluted tubule (PCT).

  • The collecting duct mainly adjusts water and electrolyte balance, but most reabsorption happens earlier, at the PCT.

2
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Which of the following processes does not occur in the renal tubules?
A) Filtration
B) Reabsorption
C) Secretion
D) Concentration of urine

🔵 Explanation:

  • Filtration happens only at the glomerulus (inside Bowman's capsule), before the renal tubules start.

  • Reabsorption, secretion, and concentration all happen inside the renal tubules (PCT, loop of Henle, DCT, and collecting duct).

3
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Process

Where it happens

Quick explanation

Filtration

ONLY at Glomerulus (Bowman's capsule)

Blood pressure forces small molecules (water, salts, glucose, urea) from blood into Bowman's capsule. This happens before the renal tubules start.

Reabsorption

In tubules (mainly PCT)

Good things like glucose, amino acids, and salts are taken back into blood from the filtrate.

Secretion

In tubules (mainly DCT)

Extra waste (like drugs, ions) is added into the filtrate from blood.

Concentration

In Loop of Henle + Collecting Duct

Water is reabsorbed to concentrate the urine.

Process

Where it happens

Quick explanation

Filtration

ONLY at Glomerulus (Bowman's capsule)

Blood pressure forces small molecules (water, salts, glucose, urea) from blood into Bowman's capsule. This happens before the renal tubules start.

Reabsorption

In tubules (mainly PCT)

Good things like glucose, amino acids, and salts are taken back into blood from the filtrate.

Secretion

In tubules (mainly DCT)

Extra waste (like drugs, ions) is added into the filtrate from blood.

Concentration

In Loop of Henle + Collecting Duct

Water is reabsorbed to concentrate the urine.

4
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Which part of the nephron is mainly responsible for concentrating the urine? A) Proximal convoluted tubule B) Distal convoluted tubule C) Loop of Henle D) Bowman's capsule

Explanation:

  • The Loop of Henle plays the main role in concentrating urine.

  • Especially the descending limb (permeable to water) and the ascending limb (permeable to ions like Na⁺ and Cl⁻ but not to water).

  • This sets up a counter-current mechanism, creating a high osmolarity in the medullary interstitium.

  • This draws water out from the filtrate later (especially at the collecting duct under the influence of ADH), concentrating the urine.

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  • ADH → Aqua-porins → Water

  • Aldosterone → Sodium

ADH (antidiuretic hormone) primarily increases the reabsorption of which substance in the kidney?
A) Urea
B) Glucose
C) Water
D) Sodium

  1. Site of Action

    • ADH acts on the collecting ducts and late distal tubules of the nephron.

  2. Mechanism

    • It binds to receptors on the cells lining these tubules.

    • This triggers insertion of aquaporin-2 water channels into their membranes.

    • Result: the tubes become highly permeable to water, so water moves out of the tubule back into the blood.

  3. Effect on Urine

    • More water is reabsorbed → less water is lost as urine → urine becomes more concentrated.

6
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In a healthy adult, the typical daily volume of urine excreted is approximately:
A) 1.5 mL
B) 15 mL
C) 1.5 L
D) 5 L

7
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Blood enters kidney via Renal Artery

Enters Glomerulus in Nephron

Step 1: Glomerular Filtration

• Blood plasma → Filtered in Bowman's Capsule

• Forms Filtrate (water, urea, glucose, salts)

Step 2: Tubular Reabsorption

• PCT: Glucose, amino acids, Na⁺, water reabsorbed

• Loop of Henle: Water (descending), Na⁺ (ascending)

• DCT: Selective reabsorption of ions, water

Step 3: Tubular Secretion

• DCT + Collecting Duct:

• Secretion of H⁺, K⁺, NH₃, drugs into filtrate

Concentration of Urine

• Loop of Henle + Vasa Recta = Countercurrent

• ADH ↑ water reabsorption in collecting duct

💧 Final Urine → Collecting Duct

Renal Pelvis → Ureter → Bladder

Excreted through Urethra (Micturition)

8
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Which part of the nephron is the primary site for tubular secretion of H⁺ and K⁺ ions?
A) Proximal convoluted tubule
B) Loop of Henle
C) Distal convoluted tubule
D) Bowman’s capsule

Explanation:

  • Tubular secretion involves the transfer of substances from the blood into the tubular fluid. The distal convoluted tubule plays a significant role in the secretion of ions, including H⁺ (protons) and K⁺ (potassium ions). This is important for regulating the body's acid-base balance and potassium levels.

  • The Loop of Henle (B) is primarily responsible for the reabsorption of water and salts, contributing to the concentration of urine, but it does not play a major role in secretion.

9
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The accumulation of urea in the blood, often indicating renal failure, is termed:
A) Glycosuria
B) Uremia
C) Albuminuria
D) Oliguria

  • Uremia refers to the accumulation of urea and other nitrogenous waste products in the blood, which often occurs due to kidney failure, as the kidneys are no longer able to filter out these wastes properly.

10
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Which of the following substances is primarily reabsorbed in the proximal convoluted tubule of the nephron?

A) Water
B) Sodium ions
C) Glucose
D) Urea

This is because glucose is almost completely reabsorbed back into the bloodstream to prevent it from being lost in the urine. The transport mechanisms in the proximal convoluted tubule are highly efficient for glucose reabsorption.

11
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Which structure prevents the backflow of urine from the urinary bladder into the ureters?
A) Sphincter muscles
B) Ureteral valves
C) Renal pelvis
D) Collecting ducts

"Urine formed by the nephrons is ultimately carried to the urinary bladder by the ureters. The bladder stores urine until a voluntary signal is given by the CNS. The stretch receptors in the bladder send signals for micturition. The oblique passage of the ureters through the bladder wall prevents the backflow of urine."

This "oblique passage" is what works like a valve to stop urine from flowing back.

12
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Which part of the nephron reabsorbs the maximum amount of glomerular filtrate?

A) Loop of Henle
B) Distal convoluted tubule
C) Proximal convoluted tubule
D) Collecting duct

The Proximal Convoluted Tubule (PCT) is the main site for reabsorption in the nephron.

  • It reabsorbs about 70-80% of the glomerular filtrate.

  • It includes glucose, amino acids, Na⁺, Cl⁻, water, etc.

  • Reabsorption here is mostly selective and active.

13
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vWhich of the following is NOT used as a biofertilizer?
A) Rhizobium
B) Azotobacter
C) Clostridium
D) Pseudomonas

Pseudomonas

  • Rhizobium: This bacterium is used as a biofertilizer, especially for leguminous plants, as it helps in nitrogen fixation by forming nodules on plant roots.

  • Azotobacter: This is also a nitrogen-fixing bacterium used as a biofertilizer.

  • Clostridium: This bacterium also aids in nitrogen fixation and is used as a biofertilizer in certain conditions.

  • Pseudomonas: This bacterium is not typically used as a biofertilizer. It is more commonly associated with plant protection as it can act as a biocontrol agent, protecting plants from pathogens.

14
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Azospirillum is commonly used as a biofertilizer for:
A) Leguminous crops only
B) Non-leguminous cereals (like wheat, rice)
C) All flowering plants
D) Fungi cultivation

B) Non-leguminous cereals (like wheat, rice).

Why B?

  • Azospirillum is a free-living, associative nitrogen-fixing bacterium that colonizes the roots of non-leguminous crops such as cereals (e.g. rice, wheat, maize).

  • It enhances their nitrogen uptake and growth, but it does not form root nodules like Rhizobium does on legumes.

15
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Blue-green algae (cyanobacteria) used as biofertilizers in paddy fields belong to the genus:
A) Nostoc
B) Anabaena
C) Azotobacter
D) Rhizopus

16
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The main benefit of using biofertilizers is that they:
A) Provide instant nitrogen to plants
B) Release nitrogen slowly and improve soil fertility
C) Eliminate the need for any fertilizers
D) Increase soil salinity

Explanation:

  • Biofertilizers are organisms (like bacteria, fungi, and algae) that enhance the nutrient availability in the soil. They help to fix atmospheric nitrogen, decompose organic matter, and improve soil structure.

  • The main advantage of biofertilizers is that they release nitrogen slowly, which enhances the soil's fertility over time and helps plants grow more sustainably. This is much better than chemical fertilizers, which can provide a quick release of nutrients but might harm the soil in the long term.

  • Biofertilizers also help in maintaining the balance of soil nutrients, unlike chemical fertilizers which can lead to soil degradation.

17
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Which part of the blastocyst is responsible for implantation into the uterine wall?
A) Inner cell mass
B) Zona pellucida
C) Trophoblast
D) Morula

Trophoblast is the correct answer.

Explanation:
The trophoblast is the outer layer of cells in the blastocyst. It helps the blastocyst attach to and invade the uterine wall, beginning the process of implantation. Later, the trophoblast gives rise to a part of the placenta.

18
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What does the inner cell mass of the blastocyst develop into?
A) Placenta
B) Amniotic sac
C) Embryo
D) Trophoblast

  • Inner cell mass (ICM) of the blastocyst develops into the embryo.

  • The trophoblast is the outer layer that helps form the placenta.

  • The amniotic sac forms later from extraembryonic structures, not directly from the ICM.

19
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Which hormone released after fertilisation maintains the corpus luteum and supports early pregnancy?

A) LH (Luteinizing hormone)
B) FSH (Follicle stimulating hormone)
C) hCG (Human chorionic gonadotropin)
D) Progesterone

C) Embryo

Explanation:

  • Inner cell mass (ICM) of the blastocyst develops into the embryo.

  • The trophoblast is the outer layer that helps form the placenta.

  • The amniotic sac forms later from extraembryonic structures, not directly from the ICM.

20
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Which of the following events occurs immediately after the sperm penetrates the ovum?
A) Cleavage
B) Implantation
C) Cortical reaction
D) Blastocyst formation

Explanation:

  • Once a sperm penetrates the ovum, the cortical reaction happens immediately. This is a defense mechanism to prevent entry of additional sperms (a process called polyspermy).

  • Only after this reaction, zygote formation occurs, followed by cleavage (cell divisions), blastocyst formation, and finally implantation.

21
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Which of the following organisms is NOT a source of restriction endonuclease?
A) Escherichia coli
B) Haemophilus influenzae
C) Streptococcus pneumoniae
D) Saccharomyces cerevisiae

Correct Answer: D) Saccharomyces cerevisiae

“The first restriction endonuclease – Hind II, was isolated and characterized from Haemophilus influenzae.”

It also mentions other restriction enzymes and their bacterial origins, such as:

  • EcoRI from Escherichia coli

  • BamHI from Bacillus amyloliquefaciens

22
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Which bacterium is the source of the thermostable DNA polymerase (Taq polymerase) used in PCR?
A) Escherichia coli
B) Thermus aquaticus
C) Bacillus subtilis
D) Pseudomonas putida

vCorrect answer: B) Thermus aquaticus

Explanation:
Taq polymerase, the enzyme used in PCR (Polymerase Chain Reaction), is derived from the bacterium Thermus aquaticus, which lives in hot springs. This enzyme can tolerate the high temperatures required for DNA denaturation in PCR, making it ideal for the process.

23
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Which of the following is commonly used as a vector in recombinant DNA technology?
A) Rhizobium
B) Salmonella typhi
C) Agrobacterium tumefaciens
D) Escherichia coli

Explanation:

  • Agrobacterium tumefaciens is a soil bacterium used as a natural genetic engineer because it can transfer part of its DNA (T-DNA from Ti plasmid) into plant genomes.

  • It's commonly used as a vector to transfer genes into plants in genetic engineering.

24
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Which of the following is true about plasmids used in genetic engineering?

A) Plasmids are found only in plants.
B) Plasmids are small, circular DNA molecules that replicate independently of chromosomal DNA.
C) Plasmids can only carry a few genes.
D) Plasmids are always harmful to the cell.

Correct! Plasmids are small, circular DNA molecules that replicate independently of chromosomal DNA. They are commonly used in genetic engineering to carry foreign genes.

25
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Which enzyme synthesizes the RNA primer required for DNA replication?
A) DNA polymerase I
B) DNA polymerase III
C) Primase
D) DNA ligase

Correct! Primase synthesizes the short RNA primer needed for DNA polymerases to start replication.

26
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Which enzyme removes RNA primers and replaces them with DNA during replication?
A) DNA polymerase I
B) DNA polymerase III
C) RNase H
D) DNA ligase

  • DNA polymerase I is responsible for removing RNA primers and replacing them with DNA during replication.

  • RNase H is involved in removing the RNA strand from an RNA-DNA hybrid molecule, but not directly involved in replacing primers.

27
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Which DNA polymerase is the main enzyme responsible for the bulk of DNA synthesis (elongation) in prokaryotes and also possesses 3′→5′ exonuclease (proofreading) activity?
A) DNA polymerase I
B) DNA polymerase II
C) DNA polymerase III
D) Primase

28
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During replication, the lagging strand is synthesized in short fragments called:
A) Okazaki fragments
B) Replication forks
C) Primer fragments
D) Telomeres

Correct! The lagging strand is synthesized in short fragments called Okazaki fragments.

Explanation:

  • The Okazaki fragments are short segments of DNA that are synthesized on the lagging strand during DNA replication.

  • These fragments are later joined together by DNA ligase to form a continuous strand.

29
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Which of the following is a biosphere reserve in India (an in situ conservation unit)?
A) Gir National Park
B) Nanda Devi
C) Bhitarkanika
D) Keoladeo National Park

Correct answer: B) Nanda Devi
Explanation:
In-situ conservation means protecting animals and plants in their natural habitat.

  • Biosphere Reserves like Nanda Devi are examples of in situ conservation.

  • Keoladeo National Park is also protected, but it's a national park, not specifically a biosphere reserve.

30
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Question:
Which of the following is an example of ex situ conservation?
A) Sacred groves
B) National parks
C) Botanical gardens
D) Wildlife sanctuaries

C) Botanical gardens

Explanation:

  • Ex situ conservation refers to protecting an endangered species by removing part of the population from a threatened habitat and placing it in a new location, which is often human-managed.
    🔹 Examples: Botanical gardens, zoological parks, seed banks, cryopreservation.

  • In situ conservation involves protecting species in their natural habitat.
    🔹 Examples: National parks, wildlife sanctuaries, biosphere reserves, and sacred groves.

31
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Each autonomic motor pathway consists of:

A) One motor neuron from CNS to effector
B) Two motor neurons in series from CNS to effector
C) Three motor neurons in series from CNS to effector
D) Only sensory neurons

Answer: B) Two motor neurons in series from CNS to effector

Explanation: ANS pathways always have a preganglionic neuron (in the CNS) synapsing at an autonomic ganglion, then a postganglionic neuron running to the effector. (NCERT Ch.21 “Autonomic Nervous System”)

32
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2. The neurotransmitter released by parasympathetic postganglionic fibers is:

A) Norepinephrine
B) Acetylcholine
C) Dopamine
D) Epinephrine

Answer: B) Acetylcholine

Explanation: Both pre- and post-ganglionic parasympathetic neurons release ACh at their synapses. (NCERT Ch.21)

33
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. The sympathetic outflow from the spinal cord arises from:

A) Cervical region only
B) Thoraco-lumbar region
C) Cranial nerves exclusively
D) Sacral region only

Answer: B) Thoraco-lumbar region

Explanation: Sympathetic preganglionic neurons originate in the thoracic and lumbar spinal segments. (NCERT Ch.21)

34
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4. “Rest and digest” functions are mediated by which division?

A) Sympathetic
B) Parasympathetic
C) Somatic
D) Enteric

Answer: B) Parasympathetic

Explanation: Parasympathetic activity lowers heart rate, stimulates digestion, and conserves energy. (NCERT Ch.21)

35
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5. Which brain region is the principal integrative center for autonomic functions?

A) Cerebellum
B) Hypothalamus
C) Midbrain
D) Medulla oblongata

Answer: B) Hypothalamus

Explanation: The hypothalamus regulates temperature, hunger, thirst, and coordinates sympathetic & parasympathetic outputs. (NCERT Ch.21 “Neural Control and Coordination”)

36
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1. In cockroach, the forewings arise from the:
A) Prothorax
B) Mesothorax
C) Metathorax
D) Abdomen

Answer: B) Mesothorax

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2. Total number of spiracles present in a cockroach is:
A) 8 pairs
B) 10 pairs
C) 2 pairs
D) 18 pairs

Answer: B) 10 pairs


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3. In cockroach, which structure stores sperms in females?
A) Mushroom gland
B) Vas deferens
C) Spermatheca
D) Colleterial gland

Answer: C) Spermatheca

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4. The type of blood vascular system in cockroach is:
A) Closed
B) Open
C) Lymphatic
D) Mixed


Answer: B) Open

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5. The number of chambers in cockroach heart is:
A) 7
B) 10
C) 13
D) 5

Answer: C) 13

41
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6. Mouthparts of cockroach are adapted for:
A) Sucking
B) Chewing and biting
C) Piercing and sucking
D) Sponging

Answer: B) Chewing and biting

42
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7. The compound eyes of cockroach are made up of about:
A) 100 ommatidia
B) 1000 ommatidia
C) 2000 ommatidia
D) 10,000 ommatidia

Answer: C) 2000 ommatidia

43
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8. Mushroom glands are found in the:
A) Male cockroach
B) Female cockroach
C) Both
D) Only in nymph

Answer: A) Male cockroach

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9. The excretory organs of cockroach are:
A) Nephridia
B) Flame cells
C) Malpighian tubules
D) Green glands

Answer: C) Malpighian tubules

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10. The development in cockroach is:
A) Complete metamorphosis
B) No metamorphosis
C) Incomplete metamorphosis
D) Direct

Answer: C) Incomplete metamorphosis (paurometabolous)

46
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11. Anal styles are found in:
A) Female only
B) Male only
C) Both
D) Absent in cockroach

Answer: B) Male only



47
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12. What is the function of antennae?
A) Respiration
B) Excretion
C) Sensory (smell and touch)
D) Reproduction

Answer: C) Sensory (smell and touch)

48
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13. In female cockroach, ootheca is formed by secretions of:
A) Spermatheca
B) Colleterial glands
C) Mushroom glands
D) Phallic gland

Answer: B) Colleterial glands