AUBF LAB PT-Module 1

microscope

Optical instrument that magnifies the size of the image of the object.

microscope

A precision instrument and is very important equipment thus, it must be kept clean and in excellent condition, mechanically and optically, at all times

Galileo Galilei and Zaccharias Janssen

The invention of this magnifying instrument was credited to _________

Anton van Leuwenhoek

Using of microscope started with whom?

Simple microscope

Has a single biconvex magnifying lens

Compound microscope

has 2 or more lenses

Compound light microscope

employs visible light as source of illumination

Compound electron microscope

source of illumination is beams of electrons

Coarse Adjustment Knob

moves the stage up and down (scanner and LPO)

Fine adjustment knob

brings the image into sharp focus ( HPO and OIO)

Base

supports the microscope

Arm

supports the body/tube and connects it to the base

Revolving nosepiece

holds the objectives and can be rotated to easily change power

Stage

horizontal platform with central opening

mechanical stage

gives controlled movement to the object on the slide

Eyepiece

part used to look through the microscope. It’s found at the top of the microscope.

Eyepiece tube

It carries the eyepiece just above the objective lens.

objective lenses

major lenses used for specimen visualization and provides the initial magnification

Nosepiece

It holds the objective lenses.

stage

the section on which the specimen is placed for viewing.

aperture

The hole on the microscope stage, through which the transmitted light from the source reaches the stage.

Rheostat control knob

Controls the intensity of light

Condenser

These are lenses that are used to collect or concentrate or focus light from the illuminator into the specimen.

Diaphragm

It’s found under the stage of the microscope and its primary role is to control the amount of light that reaches the specimen.

Upright position

How should a microscope be properly carried?

Distance of microscope from the inch of the table

1 inch

Resolution

the shortest distance between two points on a specimen that can still be distinguished by the observer or camera system as separate entities.

Magnification

amount or degree of visual enlargement of an observed object.

chromatic aberration

occur because the refractive index of every optical glass formulation varies with wavelength.

microscopic field

The areas that is visible through a microscope

Numerical aperture

measure of its ability to gather light and resolve fine specimen detail at a fixed object distance.